HISTOLOGICAL AND HISTOCHEMICAL OF MUCUS PRODUCING CELL AT SEVERAL ORGANS OF PARROT FISH (Scarus javanicus)

Cahyono Purbomartono, Akihiro Takemura, Kazunori Takano

Abstract


Mucosal secretion is the first line of defense and mucosal lining cells functions as the defensive cellular barrier against foreign agents. The mucus layer covering the body surface of teleost fishes is secreted by mucus cells (MC). In the present study, the structure of MC and its content in the epithelium of the buccal cavity, the gill arch, the esophagus and the skin of parrotfish (Scarus javanicus) were compared histologically and histochemically. A special mucus-secreting organ of the parrotfish (opercular gland) also observed using the same methods. Many MC distributed in the epithelium of buccal cavity, the esophagus and in the opercular gland of parrotfish. Many MC in the special gland of parrotfish suggest that these organs actively secrete much mucus. Contrary, lower density of MC was observed in the skin of parrotfish. Histochemical reactions revealed that out of eight lectin used in the present study. The WGA reacted widely with tissues of parrotfish in the epithelium of buccal cavity, opercular gland, gill and esophagus. These results suggests that N-'acetyl-D-glucosamine is a common carbohydrate residues of glycoproteins contained in mucus. Other lectins, PNA reacted with the skin of parrotfish. Mucus from these tissues contains carbohydrate residues of D-galactose for PNA. Mucus proteins containing these various carbohydrate residues may reveal characteristic natures of each tissues.
Keywords: histology, histochemistry, mucus, parrot fish

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