Ika Yuni Astuti


Pentagamavunon-0 has a potent anti-inflammatory activity but has poor solubility and absorption properties, which can be improved by SNEDDS formulation. One of the most critical parameters of the optimization of SNEDDS is the solubility of PGV-0 in various oils, surfactants, co-surfactant, and in the final product. This study is aimed to validate a simple and inexpensive UV-Vis spectrophotometric method for PGV-0 quantification in SNEDDS formulation. The assay was performed using UV-Vis spectrophotometer, methanol as a solvent, and Vis detection of 419 nm. The linearity, limit of detection (LOD), limit of quantity (LOQ), precision, and accuracy of the method was determined. The solubility study was performed by modified shake flask method, using labrasol as a vehicle model. The insoluble PGV-0 was separated and cleaned from the filtrate, quantified by validated UV-Vis spectrophotometry, and the solubility of PGV-0 was determined. Validation measurement was carried out in the range 1-8 ppm. The results showed a good linearity with r=0,99949, LOD=0.29 ppm, and LOQ=0.95 ppm. The accuracy expressed as recoveries were found to be 98.32 - 100.74%. The precision was good (RSD=1.29 – 1.93%). The recovery of solubility of PGV-0 was 100.02% indicated a good accuracy. This method can be used for the solubility test of many samples as in the SNEDDS formulation.


validation, UV-Vis spectrophotometry, PGV-0, solubility study


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