DETEKSI ADULTERAN PADA BAHAN BAKU SEDIAAN TEMULAWAK (Curcuma xanthorrhiza ROXB) INSTAN SECARA TLC FINGERPRINT ANALYSIS

Fauzan Zein Muttaqin, Nurul Aida, Aiyi Asnawi

Abstract


Temulawak (Curcuma xanthorrhiza Roxb) merupakan salah satu jenis tanaman unggulan yang banyak dimanfaatkan masyarakat. Pencampuran adulteran pada bahan baku sediaan temulawak dapat membahayakan kesehatan. Penelitian ini bertujuan untuk mendeteksi adulteran pada bahan baku sediaan temulawak instan. Metode yang digunakan adalah Thin Layer Chromatography (TLC) fingerprint analysis. Sidik jari KLT temulawak dibuat menggunakan rimpang temulawak yang berasal dari Cianjur, Semarang, dan Nusa Tenggara Timur. Sementara sidik jadi kunyit (Curcuma longa)sebagai adulteran utama dibuat menggunakan rimpang kunyi dari Cianjur. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Analisis kromatogram secara kemometrik menggunakan metode Principal Component Analysis (PCA). Nilai loadings Principal Component 1 (PC1) menunjukkan kurva yang linier dan data hasil scores PC1 tersebut dapat membedakan dengan baik sidik jari temulawak dari kunyit dengan nilai scores temulawak dan kunyit berada pada kuadran yang berbeda. Hasil menunjukkan bahwa nilai scores ketiga sampel temulawak instan berada di antara kuadran temulawak dan kunyit (Curcuma Longa L). Dapat disimpulkan bahwa semua sampel positif mengandung adulteran pada temulawak instan.

 

Temulawak (Curcuma xanthorrhiza Roxb) is one of the leading plant species that are widely used by the community. Adulterant mixing on raw material of temulawak preparation can be harmful. This study aims to detect adulterant on raw material of instant temulawak preparations using Thin Layer Chromatography (TLC) fingerprint analysis. TLC fingerprints of temulawak and kunyit (Curcuma longa) were made using temulawak rhizome from Cianjur, Semarang, and East Nusa Tenggara. While TLC fingerprints of kunyit (Curcuma longa) as the main adulterant were made using kunyit rhizome from Cianjur. Maceration  were carried out using ethanol 96% w/w. Analysis of chromatogram were carried out chemometrically using Principal Component Analysis (PCA) method. The loadings value of Principal Component Components 1 (PC1) showed the linear curve and the PC scores data can distinguish the temulawak fingerprint from kunyit fingerprint in different quadrants. The results showed that the score value of three instant temulawak preparation samples were located between temulawak and kunyit quadrants. It can be concluded that all samples contained kunyit as adulterant.


Keywords


Kemometrik; Kunyit (Curcuma longa L); KLT fingerprint analysis; Video Densitometri; Principal Component Analysis (PCA), Temulawak (Curcuma xanthorrhiza Roxb)

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DOI: 10.30595/pharmacy.v15i1.3059

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ISSN: 2579-910X