Optimasi Perbandingan Tween 80 dan Polietilenglikol 400 Pada Formula Self Nanoemulsifying Drug Delivery System (SNEDDS) Minyak Biji Jinten Hitam

Iis Wahyuningsih, Widyasari Putranti

Abstract


ABSTRAK Minyak biji jinten hitam (Nigella sativa Linn.), dengan kandungan utama timokuinon mempunyai aktivitas sebagai antikanker yang cukup poten. Beberapa penelitian tentang aktivitas antikanker timokuinon telah dilakukan, namun hasilnya belum maksimal. Dalam penelitian ini akan dilakukan modifikasi penghantaran self nanoemulsifying drug delivery system (SNEDDS) minyak biji jinten hitam yang diharapkan dapat meningkatkan efektivitasnya sebagai antikanker karena SNEDDS akan meningkatkan kelarutan timokuinon yang terkandung dalam minyak biji jinten hitam (MBJH), sehingga lebih banyak zat aktif yang akan sampai pada sel kanker, selain itu formula SNEDDS MBJH diduga tidak mengalami effluks oleh sel kanker. Asam oleat dipilih sebagai fase minyak, tween 80 sebagai surfaktan dan propilenglikol sebagai kosurfaktan. Tahapan penelitian yang dilakukan adalah: (1) penentuan batas bawah dan batas atas masing-masing komponen (2) optimasi formula SNEDDS MBJH menggunakan sistem DX 9 dengan parameter %T dan waktu emulsifikasi (3) validasi persamaan yang dihasilkan DX9 (4) SNEDDS hasil optimasi yang dihasilkan dikarakterisasi ukuran, zeta potensial, stabilitas termodinamiknya dan persentase transmitan menggunakan spekrofotometer. Photon correlation spectroscopy (PCS) digunakan untuk mengetahui ukuran SNEDDS dan polidispersibility index, Zeta Sizer Analysis untuk mengukur muatannya. Kata kunci: karakteristik, minyak biji jinten hitam, SNEDDS. ABSTRACT Black cumin (Nigella sativa Linn.) seed oil has been known for possessing anticancer activity, with thymoquinone as the active ingredient. Previously reported data show that anticancer activity of thymoquinone is relatively low. In this research, we modify black cumin seed oil with self nanoemulsifying drug delivery system (SNEDDS) formula, so it’s effectivity as anticancer can be improved. SNEDDS increases solubility of thymoquinonone and improves its delivery to cancer cells. We used oleic acid as oil phase, tween 80 as surfactan, and propilenglycol as cosurfactan. The steps in this research were determination of upper limit and lower limit of each components, optimization of SNEDDS formula using DX 9 system, equation validation obtained from DX 9, and characterization of SNEDDS based on its size, zeta potensial, thermodynamic stability, and thermodynamic transmitans. Key words: black cumin seeds oil, characterization, SNEDDS.

References


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