EFEK SITOTOKSIK DAN ANTIPROLIFERATIF KUERSETIN PADA SEL KANKER KOLON WiDr

Arif Wirahadi Kusuma, Nunuk Aries Nurulita, Dwi Hartanti

Abstract


Abstrak

Kanker kolon merupakan penyakit salah satu penyakit yang banyak mengakibatkan kematian. Usaha penyembuhan kanker kolon melalui pembedahan kemoterapi dan radioterapi pada umumnya belum mampu memberikan hasil yang efektif. Hal ini mengakibatkan banyak dijumpai cara pengobatan alternatif antara lain menggunakan bahan dari alam. Salah satu bahan dari alam tersebut adalah kuersetin. Kuersetin merupakan senyawa golongan flavonoid yang dikenal memiliki efek antikanker. Penelitian ini bertujuan untuk mengetahui efek sitotoksik dan antiproliferatif kuersetin terhadap sel kanker kolon WiDr. Uji sitotoksik kuersetin dilakukan menggunakan metode MTT. Hasil Uji sitotoksik menunjukkan bahwa IC50 kuersetin terhadap sel WiDr adalah 1046 μM. Pengamatan kinetika proliferasi sel WiDr yang diberi perlakuan kuersetin menggunakan metode penghitungan langsung dilakukan pada jam ke 0, 24, 48 dan 72. Pada pengamatan morfologi sel, setelah dilakukan pengecatan menunjukkan adanya fenomena apoptosis pada sel kanker kolon WiDr. Hasil penelitian menunjukkan bahwa kuersetin memiliki efek sitotoksik dan antiproliferatif pada sel kanker WiDr.

Kata Kunci : Kuercetin, WiDr, Sitotoksik, Antiproliferatif

Abstract

Colon cancer is one disease that many result in death. The of colon cancer cure through surgery chemotherapy and radiotherapy are generally not able to provide effective results. This caused many found ways to use alternative medicine among other materials from nature. One of these natural substances are quercetin. Quercetin is a flavonoid compounds are known to have anticancer effects. This study aimed to determine the effect of cytotoxic and antiproliferatif quercetin against colon cancer WiDr cells. Quercetin cytotoxic test was carried out using MTT method. Test results showed that the cytotoxic IC50 of quercetin against WiDr cells was 1046 μM. WiDr cell proliferation kinetics observation that quercetin treated using the method of direct calculation done at 0, 24, 48 and 72. In the observation of cell morphology, after the painting shows the phenomenon of apoptosis in colon cancer cell WiDr. The results showed that quercetin has cytotoxic and antiproliferative effects on cancer cells WiDr.

Keywords : Quercetin, WiDr, Cytotoxic, Antiproliferative.

References


American Cancer Society: Cancer Facts and Figures 2005 Atlanta, American Cancer Society Inc; 2005.

American Cancer Society: Cancer Statistics 2004 Atlanta, American Cancer Society Inc; 2004.

American Cancer Society. Colorectal Cancer Facts & Figures 2008-2010. Atlanta: American Cancer Society, 2008.

Andreas Gewies. 2003. Introduction to Apoptosis. ApoReview page 4.

Apantaku, L.M. 2002. Breast-conserving surgery for breast cancer. Am. Fam. Physician 66(12): 2271-2278.

Cook N, Samman S: Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources. J Nutr Biochem 1996, 7: 66-76.

Eun Jeong Choi, Su Mi Bae, and Woong Shick Ahn., 2008, Antiproliferative Effects of Kuersetin through Cell Cycle Arrest and Apoptosis in Human Breast Cancer MDA-MB-453 Cells, Arch Pharm Res Vol 31, No 10, 1281-1285.

Foster, J. S., Henley, D. C., Ahamed, S., and Wimalasena, J., 2001, Estrogen and cell cycle regulation in breast cancer, Trend in Endocrinology and Metabolism, 12(7): 320-327.

Hanahan , D., and Weinberg, R.A., 2000, The Hallmark of Cancer, Cell, 100, 57-70.

Hawariah, A.L.P. 1998. Memahami kanser. Serdang: Penerbit Universiti Putra Malaysia.

Hollman PC, van Trijp JM, Mengelers MJ, de Vries JH, Katan MB: Bioavailability of the dietary antioxidant flavonol kuersetin in man. Cancer Lett 1997, 114:139-140.

Huber W, McDaniel L, Kaderlik K, Teitel C, Lang N, Kadlubar F: Chemoprotection against the formation of colon DNA adducts from the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazol 4,5-b]pyridine (PhIP) in the rat. Mutation Res 1997, 376:115-122.

King, R.J.B., 2000, Cancer Biology, 2nd Edition, School of Biological Sciences, University of Surrey, Pearson Education, Harlow-England-London-New York, p. 150-169, 228-231, 263-264.

Kook, D., Wolf, A. H., Yu, A. L., Neubauer, A. S., Priglinger, S. G., Kampik, A., and Welge-Lüssen, U. C., The protective effect of kuersetin against oxidative stress in the human RPE in Vitro. Invest. Ophthalmol. Vis. Sci., 49, 1712-1720 (2008).

Levin B, Lieberman DA, McFarland B, Smith RA, Brooks D, Andrews KS, et al. Screening and surveillance for the early detection of colorectal cancer and adenomatous Polyps, 2008: a joint guideline from the American Cancer Society, the US Multi- Society Task Force on Colorectal Cancer, and the American College of Radiology. CA Cancer J Clin 2008;58(3):130-60.

Metodiewa, D., Jaiswal, A. K., Cenas, N., Dickancaité, E., and Segura-Aguilar, J., Kuersetin may act as a cytotoxic prooxidant after its metabolic activation to semiquinone and quinoidal product. Free Radic. Biol. Med., 26, 107-116 (1999).

Mosmann, T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays, J. Immunological Methods, 1983; 65: 55-63.

Nurulita, A. N., 2005, Efek antikanker pentagamavunon-0 (PGV-0) terhadap sel kanker payudara T47D yang diinduksi 17--estradiol melalui mekanisme induksi apoptosis dan penghambatan angiogenesis. Thesis, Program Pasca Sarjana, Universitas Gadjah Mada, Yogyakarta.

Riedl, S.J. and Shi, Y., 2004, Molecular mechanisms of caspase regulation during apoptosis, Mol. Cell Biol., 5, 897-901.

Sahu, S. C. and Gray, G. C., Pro-oxidant activity of flavonoids: Effects on glutathione and glutathione S-transferase in isolated rat liver nuclei. Cancer Lett., 104, 193-196 (1996).

Sellers, W.R., and Fisher, D.E., 1999, Apoptosis and cancer drug targeting, J. Clin. Invest., 4 (12), 1655-1661.

Schatzkin A, Freedman LS, Dawsey SM, Lanza E. Interpreting precursor studies: what polyp trials tell us about large-bowel cancer. J Natl Cancer Inst 1994;86(14):1053-7.

Tada, K, Shiho, O., Kuroshima, K., koyama, M., and Tsukamoto, K., 1986, An Improved Colometric Assay for Interleukin 2, dalam Immunological Methods, Biotechnology Laboratories, Central Research Division, Takeda chemical industries, ltd., Yodogawa-ku, Osaka, Japan.Hal 157-163

Tsao, A.S., Kim, E.S.,and Hong, W.K.,2004, Chemoprevention of Cancer, CA Cancer J. Clin., 54, 150-180.

Walaszek, Z., Hanausek, M., ang Slaga, T.J., 2004, Mechanisms of Chemoprevention, Suppl. Am. Coll. Phys., 125, 128-133.

Wyllie, A., Donahue, V., Fischer, B., Hill, D., Keesey, J., and Manzow, S., 2000, Cell death apoptosis and aecrosis, Rosche Diagnostic Corporation. Hal 1-143.

Yang CS, Landau JM, Huang MT, Newmark HL: Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr 2001, 21:381-406.

Yustina E.H., Ratih H.P., Y. Gilang Ikhtiarsyah, E.P.Septiyani dan Edy Meiyanto., 2008, Peningkatan aktivitas antiproliferatif Doxoru-bicin melalui kombinasi dengan ekstrak etanolik kulit batang waru (Hibiscus tiliaceus) terhadap sel WiDr. CCRC, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.

Zhang, J., Stanley, R. A., Adaim, A., Melton, L. D., and Skinner, M. A., 2006, Free radical scavenging and cytoprotective activities of phenolic antioxidants. Mol. Nutr. Food Res., 50, 996-1005.


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DOI: 10.30595/pji.v7i3.584

ISSN: 2579-910X