Antibacterial Activity Of Chromolaena odorata (L) King Leaves With Bioautography

Siti Rofida, Nurwahdaniati Nurwahdaniati


ABSTRACT Chromolaena odorata (L) King, commonly referred as kirinyuh, is a traditional medicinal plant from Asteraceae. It has been reported that C. odorata scientifically possessed antimicrobial activity. The aim of this study is to obtain active antibacterial substances from 70% etanolic extract of C. odorata against Staphylococcus aureus. Separation of active substances was carried out by Thin Layer Chromatography (TLC) techniques. The separated substances were tested for their antibacterial activity by bioautography assay using S. aureus. Separation of 70% etanolic extract C. odorata produced six spots with retention factor (Rf) values are 0.9, 0.8, 0.7, 0.6, 0.5, and 0.3. The diameters of inhibition zone of those spots were 35, 27, 27, 20, 31, and 14 mm, respectively. Based on the TLC profiles, the compounds with Rf of 0.9, 0.6, and 0.5 were identified as flavonoids and the compounds with Rf of 0.8, 0.7, and 0.3 were identified as terpenoids. It is concluded that the spot with the most potent antimicrobial activity was flavonoids with of Rf 0.9. Key words: antibacterial, bioautography, Chromolaena odorata.


, A.C., Ibukun, E.O., Dan-Olage, I.A. 2007. Phytochemical constituents and antioxidant properties of extract from the leaves of Chromolaena odorata. Scientific Research and Essay, 2(6):191-194. Akomas, S.C., Ijioma, S.N. 2014. Bleeding and clotting time effect of ethanolic extracts of Chromolaena odorata versus Ocimum gratissimum treated albino rats. Comprehencive Journal of Medicinal Sciences, 2 (1):9-13. Bernard, F.X., Sable, S., Cameron, B., Provost, J., Desnottes, J.F., Crouzet, J. Blanche, F. 1997. Glycosylated flavones as selective inhibitors of topoisomerase IV. Antimicrob. Agents Chemother., 41: 992-998. Choma, I.M., Grzelak, E.M., 2010. Bioautography detection in thin-layer chromatography. Journal of Chromatography A. doi:10.1016/j. chroma.2010.12.069. Debenedetti, 2009. TLC and PC. In: Sampiero, D.A., Catalan, C.A.N., Vattuone, M.A., Narwal, S.S. (Eds). Isolation, identification and characterization of allelochemicals /natural products. Enfield, USA: Sciences Publishers, pp 102-134. Harborne, J.B. 1987. Metode fitokimia: penuntun cara modern menganalisis tumbuhan (Terjemahan Kosasih, P. dan Iwang, S.), Ed 2, Bandung: Institut Teknologi Bandung. Kigigha, L.T., Zige, D.V. 2013. Activity of Chromolaena odorata on enteric and superficial etiologic bacterial agents. American Journal of Research Communication, 1 (11):266-276. Mori, A., Nishino, C., Enoki, N., Tawata, S. 1987. Antibacterial activity and mode of action of plant flavonoids against Proteus vulgaris and Staphylococcus aureus. Phytochemistry, 26: 2231–2234. Parameswari, G., Suriyavathana, M. 2012. In-Vitro antioxidant activity of Chromolaena odorata (L.) King & Robinson. International Research Journal of Pharmacy. 3(11):187-192. Reichling, J. 2010. Plant–microbe interactions and secondary metabolites with antibacterial, antifungal and antiviral properties. In: Wink, M. (Eds). Function and biotechnology of plant secondary metabolites, Ed. 2nd, Oxford: John Wiley & Sons Ltd publications, pp 214-347. Sampiero, D.A., Sgariglia, M.A., Soberon, J.R., Quiroga, E.N., Vattuone, M.A. 2009. Colorimetric Reactions. In: Sampiero, D.A., Catalan, C.A.N., Vattuone, M.A., Narwal, S.S. (Eds). Isolation, identification and characterization of allelochemicals /natural products. Enfield, USA: Sciences Publishers, pp 73-101. Stanley, M.C., Ifeanyi, O.E., Nwakaego, C., Esther, I.O. 2014. Antimicrobial effects of Chromolaena odorata on some human pathogens. International Journal of Current Microbiology and Applied Sciences, 3(3):1006-1012. Sukhthankar, J.H., Kumar, H., Godinho, M.H.S., Kumar, A. 2014. Larvacidal activity of methanolic leaf extracts of plant Chromolaena odorata L. (Asteraceae) against vector mosquitoes. International Journal of Mosquito Research, 1(3):33-38. Thodes, T.C., Bopre, M., Hallmann, J. 2007. Pyrrolizidine alkoloids of Chromolaena odorata act as nematicidal agents and reduce anifection of letuce roots by Meloidogyne incognita. Nematology, 9(3):343-349. Vaisakh, M.N., Pandey, A. 2011. The invasive weed with healing propertis: a review on Chromolaena odorata. International Journal of Pharmaceutical Sciences and Research. 3 (1):80-83. Vijayaraghavan, K., Ali, M.S., Maruthi, R. 2013. Studies on phytochemical screening and antioxidant activity of Chromolaena odorata and Annona squamosa. International Journal of Innovative Research in Sciences, Engineering and Technology, 2(12):7315-7321. Vital, P.G., Rivera, W.L. 2009. Antimicrobial activity and cytotoxicity of Chromolaena odorata (L.) King and Robinson and Uncaria perrottetii (A. Rich) Merr. extracts. Journal of Medicinal Plants Research, 3(7):511-518. Zachariades, C., Day, M., Muniappan, R., Reddy, G.V.P. 2009. Chromolaena odorata (L.) King and Robinson (Asteraceae). In: Muniappan,R., Reddy, G.V.P., Raman ,A. (Eds). Biological control of tropical weeds using artropods. Cambridge University Press. pp 130-152.

Full Text: ##PDF##

ISSN: 2579-910X