Hidah Nur Afiyah, Untung Susilo, Farida Nur Rachmawati


The activity of digestive enzymes and feed efficiency of catfish, Clarias gariepinus, wich induced by cycles of fasting and refeeding was carried out at the Laboratory of Physiology Faculty of Biology, Unsoed, Purwokerto . Experimental design with three treatments and six replications in completely randomized design (CRD) was used in this study. The treatments tested include: fish fed every day (control, P0), fish in one week were starved on Monday and Thursday and fed on the other days (P1) and fish of the week was straved two days and five days were fed a normal (P2). Catfish with average 9.57 ± 0.66 g on individual body weight, wich obtained from D3 PSDP Laboratory of Biology Faculty, Unsoed, has been used in this study. The results showed that the activity of amylase, efficiency and retention of proteins differ significantly (P <.05), but protease activity did not differ significantly (P> .05) between the treatment. Conclusion, amylase activity and feed efficiency were changes, but not for protease activity in catfish after exposed to fasting and refeeding .
Keywords: Clarias gariepinus, enzyme digestion, feed efficiency, fasting, re-feed


Amin, R., A.K.M., Bapary, M.A.J., Islam, M.S., Shahjahan, M., and M.A.R. Hossain, 2005. The Impacts of Compensatory Growth on Food Intake, Growth Rate and Efficiency of Feed Utilization in Thai Pangas (Pangasius hypothalmus). Pakistan Journal of biological Sciences. 8 (5) : 766-770.

Belanger, F., P.U. Blier and J.D. Dutil, 2003. Digestive Capacity and Compensatory Growth in Atlantic Cod (Gadus morhua). Fish Physiology and Biochemestry. 26 : 121-128.

Chan, C-R., D-N. Lee, Y-H. Cheng, D.J-Y. Hsieh and C-F. Weng, 2008. Feed Deprivation and Re-feeding on Alterations of Proteases in Tilapia, Oreochromis mossambicus. Zoological Studies, 47 (2) : 207 – 214.

Cho, S. H., S. M.Lee; B. H. Park, , S. C. Ji, J. Lee,; J. Bae, and S. Y. Oh, 2006. Compensatory growth of Juvenile Olive Flounder, Paralichthys olivaceus, L., and Changes in Proximate Composition and Body Condition Indices during Fasting and after Refeeding in Summer Season. Journal of the World Aquaculture Society. 37, 2:168-174.

Eroldoğan, O.T., C. Suzer, O. Taşbozan, and S. Tabakoğlu, 2008. The Effects of Rate-Restricted Feeding Regimes in Cycles on Digestive Enzymes of Gilthead Sea-bream, Sparus aurata. Turkish Journal of Fisheries and Aquatic Sciences. 8 : 49 – 54.

Furne, M., G.M. Gallego, M.C. Hidalgo, A.E. Morales , A. Domezain, J. Domezaine and A. Sanz, 2008. Effect of Starvation and Refeeding on Digestive Enzyme Activities in Sturgeon (Acipencer naccari) and Trout (Oncorhynchus mykiss). Compararative Biochemistry and Physiology. Part A. 149(4) : 420-425.

Haiqing, S. and H. Xiqin, 1994. Effect of Dietary Animal and Plant Protein Ratios and Energy Levels on Growth and Body Composition of Bream, Megalobrama skolkovii Dybowski, Fingerlings. Aquaculture 127 : 189 -196.

Hidalgo, M.C., E. Urea, and A. Sanz, 1999. Comparative Study of Digestive Enzymes in Fish with Different Nutritional Habits. Proteolytic and Amylase Activities. Aquaculture. 170 : 267 – 283.

Steel, R.G.D. and J.H. Torrie, 1981. Principles and Procedures of Statistic a Biometrical Approach2 nd. Mc Graw Hill Book Company, Singapore.

Susilo, U., E. Yuwono dan F.N. Rachmawati, 2009. Status Fisiologi pada Pertumbuhan Kompensatori yang Diinduksi Dengan Pemuasaan Secara Periodik Untuk Optimasi Produksi Ikan Patin (Pangasius hypothalmus). Laporan Penelitian. Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto.

Turano, M.J., R.J. Borski, and H.V. Daniels, 2007. Compensatory Growth of Pond Reared Hybrid Striped Bass, Morone chrysops x Morone saxatilis, Fingerling. Journal of the World Aquaculture Society, 38 (2): 250-261.

Uys, W., T. Hecht and M. Walter, 1987. Changes in Digestive Enzyme Activities of Clarias gariepinus (Pisces : Claridae) After Feeding. Aquaculture. 63(1-4): 243-250.

Watanabe, W.O., S.C. Ellis and J. Chaves, 2001. Effects of Dietary Lipid and Energy to Protein Ration on Growth and Feed Utilization of Juvenile Mutton Snapper, Lutjanus analis fed Isonitrogenous Diets at Two Temperatures. Journal of The Aquaculture Sociaty. 32 (1) : 30-40.

Yuwono, E., P. Sukardi dan U. Susilo, 2008. Kondisi Fisiologis pada Pertumbuhan Kompensatori yang Diinduksi dengan pembatasan Pakan Sebagai Upaya Optimalisasi Produksi Ikan Gurami. Laporan Penelitian. Fakultas Biologi Unsoed, Purwokerto.

Full Text: ##PDF##

ISSN: 1410-94255