Optimasi Perbandingan Tween 80 dan Polietilenglikol 400 Pada Formula Self Nanoemulsifying Drug Delivery System (SNEDDS) Minyak Biji Jinten Hitam

Iis Wahyuningsih; Widyasari Putranti

Optimasi Perbandingan Tween 80 dan Polietilenglikol 400 Pada Formula Self Nanoemulsifying Drug Delivery System (SNEDDS) Minyak Biji Jinten Hitam

Iis Wahyuningsih, Widyasari Putranti

Abstract

ABSTRAK Minyak biji jinten hitam (Nigella sativa Linn.), dengan kandungan utama timokuinon mempunyai aktivitas sebagai antikanker yang cukup poten. Beberapa penelitian tentang aktivitas antikanker timokuinon telah dilakukan, namun hasilnya belum maksimal. Dalam penelitian ini akan dilakukan modifikasi penghantaran self nanoemulsifying drug delivery system (SNEDDS) minyak biji jinten hitam yang diharapkan dapat meningkatkan efektivitasnya sebagai antikanker karena SNEDDS akan meningkatkan kelarutan timokuinon yang terkandung dalam minyak biji jinten hitam (MBJH), sehingga lebih banyak zat aktif yang akan sampai pada sel kanker, selain itu formula SNEDDS MBJH diduga tidak mengalami effluks oleh sel kanker. Asam oleat dipilih sebagai fase minyak, tween 80 sebagai surfaktan dan propilenglikol sebagai kosurfaktan. Tahapan penelitian yang dilakukan adalah: (1) penentuan batas bawah dan batas atas masing-masing komponen (2) optimasi formula SNEDDS MBJH menggunakan sistem DX 9 dengan parameter %T dan waktu emulsifikasi (3) validasi persamaan yang dihasilkan DX9 (4) SNEDDS hasil optimasi yang dihasilkan dikarakterisasi ukuran, zeta potensial, stabilitas termodinamiknya dan persentase transmitan menggunakan spekrofotometer. Photon correlation spectroscopy (PCS) digunakan untuk mengetahui ukuran SNEDDS dan polidispersibility index, Zeta Sizer Analysis untuk mengukur muatannya. Kata kunci: karakteristik, minyak biji jinten hitam, SNEDDS. ABSTRACT Black cumin (Nigella sativa Linn.) seed oil has been known for possessing anticancer activity, with thymoquinone as the active ingredient. Previously reported data show that anticancer activity of thymoquinone is relatively low. In this research, we modify black cumin seed oil with self nanoemulsifying drug delivery system (SNEDDS) formula, so it’s effectivity as anticancer can be improved. SNEDDS increases solubility of thymoquinonone and improves its delivery to cancer cells. We used oleic acid as oil phase, tween 80 as surfactan, and propilenglycol as cosurfactan. The steps in this research were determination of upper limit and lower limit of each components, optimization of SNEDDS formula using DX 9 system, equation validation obtained from DX 9, and characterization of SNEDDS based on its size, zeta potensial, thermodynamic stability, and thermodynamic transmitans. Key words: black cumin seeds oil, characterization, SNEDDS.

References

Fisher Scientific. 2014. http://www.fishersci.com/ecomm/servlet/fsproductdetail_10652_667905__-1_0. El-Mahdy, M.A. 2005, Thymoquinone induces apoptosis through activation of caspase-8 and mitochondrial events in P53-Null myeloblastic leukemia HL-60 cells. International Journal of Cancer, 117(3):409-417. Date, A.A. dan Nagarsenker, M.S. 2007. Design and evaluation of self-nanoemulsifying drug systems (SNEDDS) for cefpodoxime proxetil. International Journal of Pharmaceutics, 392:166-172. Dankort, D.l. dan Muller, W.J. 2000. Signal transduction in mammary tumorigenesis: a transgenic prespektive. Oncogene, 19:1038-44. Deng, C. dan Scott, F. 2000. Role of the tumor suppressor Brcal in genetic stability and mammary gland tumor formation. Oncogene, 19:1059-64. Gali-Muhtasib, H., Roessner, A. dan Schneider-Stock, R. 2006. Thymoquinone: A promising anti-cancer drug from natural sources. The International Journal of Biochemistry & Cell Biology, 38(8):1249-1253. Hugger, E.D., Audus, K.L., dan Borchardt, R.T. 2002. Effects of poly (ethylene glycol) on efflux transporter activity in Caco-2 cell monolayers. J. Pharm. Sci., 91:1980-1990. Ivankovic, S. 2006. The antitumor activity of thymoquinone and thymohydroquinone in vitro and in vivo. Experimental Oncology, 28(3):220-224. Jemal, A., Center, M.M., DeSantis, C., Ward, E., Murray, T., Xu, J., Thun, M.J. 2007. Cancer statistic 2007. CA Cancer J Clin., 57:43-66. Kubatka, P., Ahlersova, E., Ahlers, I., Bojkova, B., Kalicka, K., Adamekova, E., Markova, M., Chamilova, M., dan Cermakova, M. 2002. Variability of mammary carsiogenesis induction in female Sprague-Dawley and Wistar: Han rats: the effects of season and age. Physiol. Res., 51:633-40. Massadeh, A.M., Al-Safi, S.A., Momani, I.F., Al-Mahmoud, M., Alkofahi, A.S.,2007. Analysis of cadmium and lead in mice organs: effect of Nigella sativa L. (black cumin) on the distribution and immunosuppressive effect cadmium-lead mixture in mice. Biol Trace Elem Res., 115(2):157-67. Mbarek, L.A., Ait Mouse, H., Elabbadi, H.I., Bensalah, M., Gamouh, A., Aboufatima, R., Benharref, A., Chait, A., Kamal, M., Dalal, A., dan Zyad, A. 2007. Anti-tumor properties of blackseed (Nigella sativa L.) extracts. Braz J Med Biol Res., 40(6):839-47. Mechanic, L.E., Marrogi, A.G., Welsh, J.A., Bowman, E.D., Khan, M.A., Enewlod, L., Yun-Ling Zheng, Chanock, S., Shields, P.G., dan Harris, C.C. 2005. Polymorphism in XOD and TP53 and mutation in human lung cancer. Carcinogenesis, 26(3):597-604. Nickavar, B., Mojab, F., Javidnia, K., Amoli, M.,A. 2003. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. J. Naturforsch, 58(9-10):629-31. Patel, J., Kevin, G., Patel, A., Raval, M., dan Sheth, N. 2012. Design and development of a self-nanoemulsifying drug delivery system for telmisartan for oral drug delivery. International Journal of Pharmaceutical Investigation, 1:112-118. Purnomosari, D., Paramita, D.K., Aryandono, T., Pals, G., Van Diest, P.J. 2005. A novel BRCA2 mutation in an Indonesian family found with a new, rapid, and sensitive mutation detection method based on pooled denaturing gradient gel electrophoresis and targeted sequencing. J Clin Pathol., 58:493-99. Tjindarbumi, D. dan Mangunkusumo, R. 2002. Cancer in Indonesia, present and future. Jpn. J. Clin. Oncol., 32(suppl 1):S17-S21. Yi, L., Hively, W.P., Varmus, H.E. 2000. Use of MMTV-Wnt-1 transgenic mice for studying the genetic basis of breast cancer, Oncogene, 19:1002-9.

ISSN: 2579-910X