Potential Use of Clove Oil Diluted in VCO On Contaminated Wound Healing
Abstract
Essential oils, including clove oil, has been used broadly through human civilization due to its multiple benefits, including its utilization as natural antiseptics. In spite of its benefits, clove oil can cause irritation if applied neat to the skin, thus a carrier oil is needed for topical application of clove oil. Clove oil together with virgin coconut oil as a carrier, has potential synergic benefit if applied on contaminated wounds due to its enhanced antiseptic and anti-inflammatory properties. The aim of this study is to investigate the effect of topical application of clove (Syzygium aromaticum) oil diluted in Virgin Coconut Oil on contaminated wound. This study was a quasi-experimental research with post-test only control group design. Clove oil that had been diluted in VCO was applied on contaminated excisional wound in white male rat wistar strain once daily. The wound was inflicted using unsterilized scalpel. The wound was then left exposed to the environment without any dressing and the healing process was then evaluated by measuring the wound area percentage once every four days for sixteen days. Friedman analysis used in this study showed a significant result (p<0,001) and Concentration of 5% clove oil diluted in VCO was found to be the most effective concentration based on the measurement of wound area percentage on the last day of measurement. This study concludes that the topical application of clove oil diluted in VCO showed a significant difference in wound healing process.
Keywords
References
1. Hesham H. A. Rassem, Abdurahman h. nour rosli m. yunus. Techniques For Extraction of Essential Oils From Plants : A Review. Aust J basic Appl Sci. 2016;10(November):117-127.
2. W. Dhifi, S. Bellili, S. Jazi, N B and WM. Essential Oils ’ Chemical Characterization and Investigation of Some Biological Activities : Medicines. 2016;3(4):25. doi:10.3390/medicines3040025
3. Sorg H, Tilkorn DJ, Hager S, Hauser J, Mirastschijski U. Skin Wound Healing: An Update on the Current Knowledge and Concepts. Eur Surg Res. 2017;58(1-2):81-94. doi:10.1159/000454919
4. Kesici S, Kesici U, Ulusoy H, Erturkuner P, Turkmen A, Arda O. Effects of local anesthetics on wound healing. Brazilian J Anesthesiol (English Ed. 2018;68(4):375-382. doi:10.1016/j.bjane.2018.01.020
5. Mimica-dukić N, Orčić D, Lesjak M, Šibul F. Essential Oils as Powerful Antioxidants : Misconception or Scientific Fact ? Published online 2016.
6. Nurdjannah N. Diversifikasi Penggunaan Cengkeh. Perspekt Rev Penelit Tanam Ind. 2006;(12).
7. Bhowmik D, Kumar KPS, Yadav A, Srivastava S, Paswan S, Dutta AS. Recent Trends in Indian Traditional Herbs Syzygium Aromaticum and its Health Benefits. J Pharmacogn Phytochem. 2012;1(1):6-17.
8. Uddin MA, Shahinuzzaman M RM and YZ. Study of chemical composition and medicinal properties of volatile oil from clove buds (Eugenia caryophyllus). Int J Pharm Sci Res 2017. 2017;6(2):5-9. doi:10.13040/IJPSR.0975-8232.8(2).895-99
9. Orchard A, Vuuren S Van. Commercial Essential Oils as Potential Antimicrobials to Treat Skin Diseases. 2017;2017. doi:10.1155/2017/4517971
10. van Koppen CJ, Hartmann RW. Advances in the treatment of chronic wounds: a patent review. Expert Opin Ther Pat. 2015;25(8):931-937. doi:10.1517/13543776.2015.1045879
11. Alam P, Ansari MJ, Anwer MK, Raish M, Kamal YKT, Shakeel F. Wound healing effects of nanoemulsion containing clove essential oil. Artif Cells, Nanomedicine, Biotechnol. 2017;45(3):591-597. doi:10.3109/21691401.2016.1163716
12. Orchard A, Kamatou G, Viljoen AM, Patel N, Mawela P, van Vuuren SF. The Influence of Carrier Oils on the Antimicrobial Activity and Cytotoxicity of Essential Oils. Evid Based Complement Alternat Med. 2019;2019:6981305. doi:10.1155/2019/6981305
13. Varma SR, Sivaprakasam TO, Arumugam I, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J Tradit Complement Med. 2018;9(1):5-14. Published 2018 Jan 17. doi:10.1016/j.jtcme.2017.06.012
14. Viljoen JM, Cowley A, Du Preez J, Gerber M, Du Plessis J. Penetration enhancing effects of selected natural oils utilized in topical dosage forms. Drug Dev Ind Pharm. 2015;41(12):2045-2054. doi:10.3109/03639045.2015.1047847
15. Evangelista MTP, Abad-Casintahan F, Lopez-Villafuerte L. The effect of topical virgin coconut oil on SCORAD index, transepidermal water loss, and skin capacitance in mild to moderate pediatric atopic dermatitis: a randomized, double-blind, clinical trial. Int J Dermatol. 2014;53(1):100-108. doi:10.1111/ijd.12339
16. Varma SR, Sivaprakasam TO, Arumugam I, et al. In vitro anti-inflammatory and skin protective properties of Virgin coconut oil. J Tradit Complement Med. 2018;9(1):5-14. doi:10.1016/j.jtcme.2017.06.012
17. A. Trattner, M. David and AL. Occupational contact dermatitis due to essential oils. In: Contact Dermatitis, Vol. 58, No. 5. ; 2008:282–284.
18. Singh S, Young A, McNaught CE. The physiology of wound healing. Surg (United Kingdom). 2017;35(9):473-477. doi:10.1016/j.mpsur.2017.06.004
19. Pérez-Rosés R, Risco E, Vila R, Peñalver P, Cañigueral S. Biological and Nonbiological Antioxidant Activity of Some Essential Oils. J Agric Food Chem. 2016;64(23):4716-4724. doi:10.1021/acs.jafc.6b00986
20. Gonzalez AC de O, Costa TF, Andrade Z de A, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016;91(5):614-620. doi:10.1590/abd1806-4841.20164741
21. Caley MP, Martins VLC, O’Toole EA. Metalloproteinases and Wound Healing. Adv wound care. 2015;4(4):225-234. doi:10.1089/wound.2014.0581
22. auf dem Keller U, Sabino F. Matrix metalloproteinases in impaired wound healing. Met Med. Published online May 26, 2015:1. doi:10.2147/MNM.S68420
23. Nam H, Kim M-M. Eugenol with antioxidant activity inhibits MMP-9 related to metastasis in human fibrosarcoma cells. Food Chem Toxicol. 2013;55:106-112. doi:10.1016/j.fct.2012.12.050
24. Tsai T-H, Huang W-C, Lien T-J, et al. Clove extract and eugenol suppress inflammatory responses elicited by Propionibacterium acnes in vitro and in vivo. Food Agric Immunol. 2017;28(5):916-931. doi:10.1080/09540105.2017.1320357
DOI: 10.30595/hmj.v6i1.17356
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN: 2620-567X
