AKTIVITAS ANTIOKSIDAN EKSTRAK PERASAN DAUN MANGGIS (Garcinia mangostana L.) BERDASARKAN METODE DPPH (2,2 Diphenyl-1-phycryl hydrazil)
DOI:
https://doi.org/10.30595/pji.v9i3.762Abstract
ABSTRAK Garcinia mangostana L merupakan salah satu tanaman yang mempunyai daya antioksidan alami yang diketahui mampu menghambat radikal bebas.Penelitian ini bertujuan untuk Membuktikan kemampuan aktivitas antioksidan dari Ekstrak perasan daun manggis (Garcinia mangostana, L), perbandingan aktivitas antioksidan antara perasan daun manggis dengan kontrol positif. konsentrasi suatu zat di dalam perasan daun manggisditentukan dengan metode DPPH Menggunakan spektrofotometri UV-Vis. Aktivitas antioksidan ekstrak perasan daun manggis menggunakan parameter Inhibition Concentration (IC50) yang dapat menyebabkan 50% DPPH kehilangan karakter radikal. Aktivitas antioksidan ekstrak perasan daun manggis memiliki IC50 sebesar 19,3708 ppm hal ini menunjukkan bahwa pada konsentrasi tersebut memiliki penghambatan 50% aktivitas radikal bebas DPPH, dan Kontrol positif vitamin E memiliki IC50 sebesar 2,146 ppm. Hasil ini menunjukkan ekstrak perasan daun manggis lebih rendah 9 kali dari daya antioksidan kontrol positif. Berdasarkan hasil Uji T menunjukkan bahwaekstrak perasan daun Manggis dan Kontrol positif berbeda.Dan kemampuan antioksidan ekstrak perasan daun manggis sangat kuat dengan nilai IC50 kurang dari 50. Kata kunci : Daun manggis, perasan, antioksidan, DPPH ABSTRACT Garcinia mangostana L is one of the main power plant that has natural antioxidants that are known to inhibit the scavenging free radicals.This study aims to Prove the ability of the antioxidant activity leaf juice extract of mangosteen leaves (Garcinia mangostana, L), the ratio of antioxidant activity between extract juice of mangosteen leaveswith positif control. Concentration of a substance in the juice extract of mangosteen leaves is determined by the DPPH method using UV-Vis spectrophotometry. It aims to determine the antioxidant activity of extract juice of mangosteen leaves using parameters Inhibition Concentration (IC50) which can cause loss of 50% DPPH as free radical. .The antioxidant activity of extract juice of mangosteen leaves has IC50 of 19.3708 ppm it shows that at these concentrations has 50% inhibition of DPPH free radical activity, and the vitamin E has a positive control ofa positivencontrol C50 2,146 ppm.These results indicate mangosteen juice extract of mangosteen leaves is lower than 9 times the antioxidant activity of a positive control. Based on the results of T test showed that extract juice of the mangosteen leaves and positive controls are different. Antioxidant activity from juice extract of mangosteen leaves is very strong with IC50 values of less than 50. Key words : Mangosteen leaves, juice ,antioxidant, DPPHReferences
Amar, 2011, Uji Aktivitas Antioksidan Ekstrak Etanol Daun Manggis Dengan Metode DPPH, jurnal ilmiah farmasi, Universitas Muhammadiyah Purwokerto.
Gulluce M, Aslan A, Sokmen M, Sahin F, Adiguzel A, Agar A, Sokmen A, 2006. Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatis glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana, J.Phytomedicine 13(2006), p.515-521
Harborne JB, 1987. Metode Fitokimia. (Terjemahan).Kosasih Padmawinata. Bandung : Penerbit ITB.
Huang, D. J., Chen, H.J., Lin, C. D., Lin, Y. W., 2005, Antioxidant and antiproliverative activities of water spinach (Ipomoea aquatica Forsk) constituents, Bot. Bull. Acad. Sin. (2005) 46: 99-106
Mardawati, Filiantiy F., Marta, H. 2010, Jurnal Kajian Aktivitas Antioksidan Ekstrak kulit manggis (Garcinia mangostanaL) Dalam Rangka Pemanfaatan Limbah Kulit Manggis Di Kecamatan Puspahiang KabupatenTasikmalaya, Bandung:Universitas Padjajaran.
Subroto, 2009.Minuman Temulawak. http://ebooksolusi.blogspot.com/search/minumantemulawak(Diakses tanggal 1Februari 2010).
Surnani,T,2005, â€Aktivitas Antioksidan Penangkap Radikal Bebaas Beberapa Kecambah Dari Biji Tanaman FamilI Papilionaceaeâ€,Jurnal Farmasi Indonesia4,34-39
Winarsi, H. 2007. Antioksidan Alami & Radikal Bebas. Kanisius. Yogyakarta : 11-20, 79-82, 147-161.
Windono, T., Hendrajaya, K., Nurfatmawati, H., Soraya, F., 2004, Pengaruh Cara Pengeringan Daun Dewa (Gynura Pseudo-China L.) Terhadap Kapasitas Peredam Radikal Bebas dari Ekstrak Metanol Simplisianya pada 1,1-Diphenyl 1-2-Picrylhidrazyl (DPPH), Artocarpus, Vol 4 (1), 27-31
.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
