Unlocking the Potential of Nano Husk Ash in Enhancing Trichoderma's Efficacy against Fusarium spp. Causal Twisted Disease: An In Vitro Investigation

Taufiq Hidayat; Ragga Dony Santoso; Khoirudin Khoirudin; Siti Nur Aisyah; Mulyono Mulyono

doi:10.30595/agritech.v27i2.17888

Authors

Taufiq Hidayat Universitas Muhammadiyah Yogyakarta
Ragga Dony Santoso Universitas Muhammadiyah Yogyakarta
Khoirudin Khoirudin Universitas Muhammadiyah Yogyakarta
Siti Nur Aisyah Universitas Muhammadiyah Yogyakarta
Mulyono Mulyono Universitas Muhammadiyah Yogyakarta

DOI:

https://doi.org/10.30595/agritech.v27i2.17888

Keywords:

Biological control, Inhibition mechanism, Nano rice hush ash, Twisted disease

Abstract

Shallots, a vulnerable vegetable crop, are often affected by twisted disease caused by Fusarium spp., Enhancing plant resistance is crucial, and one approach is the utilization of biological agents like Trichoderma sp. combined with rice husk ash nanoparticles. This study aimed to assess the efficacy of rice husk ash nanoparticles and Trichoderma sp. in controlling Fusarium spp. causing twisted disease in shallots under in vitro conditions. The dual culture method was employed, where Fusarium spp. and Trichoderma sp. were co-cultured on Potato Dextrose Agar (PDA) with a 3 cm spacing, while nano husk as combine with the PDA . The experimental design was a completely randomized design with three treatments: Nano Rice husk ash with concentration 0.3%, Trichoderma sp., and combination between nano rice husk ash and Trichoderma sp.. The variables assessed included the characteristics of Trichoderma sp. and Fusarium spp., as well as the percentage of inhibition. Data were analyzed using analysis of variance (Î± = 5%). The results demonstrated that the combined application of nano rice husk ash with concentration 0.3% and Trichoderma sp. exhibited the highest resistance percentage, reaching up to 90%. The inhibition mechanism involved competition and antibiosis exerted by the Trichoderma sp. against Fusarium spp..Â This finding suggests a promising approach for controlling Fusarium-induced twisted disease in shallots.

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