Genotype-Dependent Optimization of In Vitro Regeneration and Phytohormone Responses in Sugarcane

Authors

  • Ghulam Farooque Department of Biotechnology, Sindh Agriculture University Tandojam, Pakistan
  • Arfa University of Sindh Jamshoro, Sindh, Pakistan

DOI:

https://doi.org/10.30595/agritech.v28i1.28313

Keywords:

Genotype-specific responses, Micropropagation, Plant regeneration, Phytohormone, Saccharum officinarum

Abstract

In vitro regeneration plays an essential role in developing crop improvement through targeted use of phytohormones. The experiment was carried out at the Nuclear Institute of Agriculture, Tando Jam, and investigated the genotypic variability in the in vitro regeneration of three sugarcanes (Saccharum Officinarum L.) genotypes (BL4, Thatta-10, NIA-2004) on phytohormone-supplemented Murashige and Skoog (MS) medium to optimize propagation protocols. BL4 showed the highest callus induction (4.97 g) on MS with 5.0 mg/L 2,4‑dichlorophenoxy acetic acid (2,4‑D), while NIA-2004 exhibited the lowest. Regeneration efficiency was greatest for NIA-2004 (28.11 plantlets) on media with 3.0 mg/L indole‑3‑acetic acid (IAA), indole‑3‑butyric acid (IBA), and kinetin, whereas BL4 produced the tallest shoots (14.0 cm) on the same medium. Rooting performance also varied; Thatta-10 produced the most roots (25.11 plantlets) and NIA-2004 the longest roots (7.52 cm) on ½-strength MS with 2.0 mg/L IBA. These results demonstrate genotype-specific phytohormone sensitivity, underscoring the need for customized protocols to develop elite, disease-free cultivars.

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Published

2026-06-01

Issue

Vol. 28 No. 1 (2026): AGRITECH

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Articles

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Copyright (c) 2026 Ghulam Farooque, Arfa

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