PENGARUH BERBAGAI JENIS STERILAN DAN WAKTU PERENDAMAN TERHADAP KEBERHASILAN STERILISASI EKSPLAN DAUN KENCUR (Kaempferia galanga L) PADA TEKNIK KULTUR IN VITRO
DOI:
https://doi.org/10.30595/agritech.v22i1.7523Abstract
This study aims to determine the effect of sterile and immersion duration on the success of sterilization of kencur (Kaempferia galanga L) leaf explants using in vitro culture techniques and to find out the types of contaminants that emerge. The study was conducted from October 2018 to February 2019, located at the Basic Agrotechnology Laboratory and Plant Engineering Laboratory, Faculty of Agriculture, Universitas Muhammadiyah Purwokerto. The design used was a randomized block design (RBD) with 15 treatments. Each treatment was repeated 3 times. The treatments were the immersed in 6% chlorine for 10 minutes (S1), for 15 minutes (S2), for 20 minutes (S3), the immersed in 0,1 g/ml HgCl2 for 1 minute (S4), for 3 minutes (S5), for 5 minutes (S6), the immersed in HgCl2 0,2 g/ml for 1 minute (S7), for 3 minutes (S8), for 5 minutes (S9), the immersed in HgCl2 0,3 g/ml for 1 minute (S10), for 3 minutes (S11), 5 minutes (S12), immersed in dithane 2 g/l for 1 hour (S13), for 12 hours (S14), for 24 hours (S15). The results showed that the treatment of Dithane sterile 2 g/l water with an immersion duration of 1 hour (S13) significantly affected the percentage of contamination and succeeded in reducing contamination by 44.44%, while the use of chlorine sterile and HgCl2 had no significant effect. The types of contaminants that appear are Macrophomoina sp., Aspergillus sp., Cladosporium sp., And Pseudomonas sp.
References
Adji, Dhirgo. 2007. Perbandingan Efektivitas Sterilisasi Alkohol 70%, Inframerah, Otoklaf, dan Ozon terhadap Pertumbuhan Bakteri Bacillus subtilis. Jurnal Sain Veteriner. Vol 25, No 1. Hlm. 17-24.
Alfian Z. 2006. Merkuri: Antara Manfaat Dan Efek Penggunaannya Bagi Kesehatan Manusia Dan Lingkungan. Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Sumatera Utara, Medan
Crous PW, Schroers H-J, Groenewald JZ, Braun U, Schubert K. 2005. Metulocladosporiella gen. nov. for the casual organism of Cladosporium speckle disease of banana. Mycological Research 110:256-275. DOI :10.1016
Darmono,D.W.2003.Menghasilkan Anggrek Silangan. Jakarta : Penebar Swadaya.
Djojosumarto, P. 2004. Teknik Aplikasi Pestisida Pertanian. Kanisius, Yogyakarta. 211 p.
Estrela, C., Barbin E.L., Spano J.C., Marchesan, M.A., 2002. Mechanism of action of sodium Hypochlorite.BrazDentJ.13(2):113-117.
Fauzan, Y.S.A, Supriyanto, Tajuddin T. 2017. Efektivitas Merkuri Klorida (HgCl2) Pada Sterilisasi Tunas Samping Jati (Tectona Grandis) In Vitro. J Bioteknol Biosains Indones – Vol 4 No 2 Thn 2017
34
Farooq SA, Farooq TT, Rao TV (2002) Micropropagation of Annona squamosa L. using nodal explants. Pakistan Journal of Biological Sciences 5(1): 43-46.
Gunawan LW. 1987. Teknik Kultur Jaringan. Lembaga Sumberdaya Informasi IPB. Bogor : Laboratorium Kultur Jaringan Tanaman Pusat Antar Universitas Bioteknologi IPB
Gunawan LW (1992) Teknik Kultur Jaringan Tanaman. PAU Bioteknologi. Departemen Pendidikan dan Kebudayaan. Direktorat Jenderal Pendidikan Tinggi. Institut Pertanian Bogor, Bogor
Habibah, NA, Sumadi, Ambar S (2013) Optimasi Sterilisasi Permukaan Daun dan Eliminasi Endofit pada Burahol. Journal of Biology & Biology Education Biosaintifika 5 (2) : 95 – 99
Hadioetomo, Katna S., Imas T., Tjitrosono S.S., Angka S.L. 2005. Dasar-Dasar Mikrobiologi 1. UI, Jakarta.
Hidayat N. M.C. Padaga, Suharti S. 2006. Mikrobiologi Industri. Penerbit Andi, Yogyakarta.
Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley, S. T. Wiliam. 1994.BergeysManualofDeterminative Bacteriology 9th ed. Lippincott Wiliamand Wikins. Philadelphia. 785 hal
Ilyas, S. 2001. Mikrobiologi Dasar Diklat Kompilasi 28. Universitas Sumatra Utara Press, Medan
Lestari E.G. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen 7 (1).
Pancaningtyas, S. dan C. Ismayadi. 2001. Sterilisasi Ulang pada Perbanyakan Somatic Embryogenesis Kakao (Theobromo cacao L.) untuk PenyelamatanEmbrioTerkontaminasi. Pelita Perkebunan 27(1).
Priadi D, Fitriani H, Sudarmonowati E. 2008. Pertumbuhan in vitro Tunas Ubi Kayu (Manihot esculenta Crantz) pada Berbagai Bahan Pemadat Alternatif Pengganti Agar. Biodiversitas. Vol. 9 No. 1 Hal. 9-12.
Rahmat R. 2004. Temu-Temuan Apotik Hidup Di Pekarangan. Kanisius : Yogyakarta.
Samson A.R dan Reenen Hoekstra ES van. 1988. Introduction to Food Borne Fungi. Centralbureau Voor Schimmelcultures. Baarn. Delpt.
Sandra, E.2002. Membuat Anggrek Rajin Berbunga. Jakarta: Agro Media Pustaka. Jakarta.
Savitri, S.D.N. 2006. Isolasi dan Karakteristik Bakteri Halotoleran Pada Peda Ikan Kembung (Rastrellinger sp.). Skripsi. IPB, Bogor.
Shofiyani, A., O.D. Hajoeningtijas. 2010. Pengaruh Sterilan Dan Waktu Perendaman Pada Eksplan Daun Kencur ( Kaemferia galanga L) Untuk Meningkatkan Keberhasilan Kultur Kalus. AGRITECH, Vol. XII No. 1 Juni 2010 : 11 – 29
Suratman, Pitoyo, A. Mulyani, S. 2013. Keefektifan Penggunaan Bahan Sterilisasi Dalam pengendalian Kontaminasi Eksplan Pada Perbanyakan tanaman Sirsak (Annona Muricata L.) Secara In Vitro. Jurnal Jurusan Biologi FMIPA UNS Surakarta
Syamsuhidayat, S.S. dan Johny, R.H,. 1991. Inventaris Tanaman Obat Indonesia (1).Jakarta: Bakti Husada. hal 596-7
Tulainy, I. 2016. pengaruh auksin (2,4 d) dan air kelapa terhadap induksi kalus pada rimpang kencur (Kaempferia galanga L). Skripsi. UMP.
Watanabe, T, 2002. Pictorial Atlas of Soil and Seed Fungi. CRC Press, New York Wolf, J.B. 2007. Tissue Culture Methods. Departement of Biological Sciences, University of Maryland. Baltimore Country 1000 Hilltop Circle Baltimore Md 21250.
Wudianto, R. 2002. Petunjuk Penggunan Pestisida. Jakarta: Penebar Swadaya.
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