Eksplorasi Ekstrak Etanol Beberapa Tumbuhan Berpotensi Sebagai Antiketombe
DOI:
https://doi.org/10.30595/jrst.v1i2.1671Keywords:
antifungi, ekstrak etanol, ketombeAbstract
AbstrakÂ
Ketombe merupakan salah satu masalah rambut yang disebabkan oleh fungi Malassezia furfur sehingga untuk pengendaliannya diperlukan agen antifungi. Beberapa tumbuhan telah digunakan secara empiris untuk terapi antiketombe antara lain: inggu (Ruta angustifolia), bambu tali (Gigantochloa apus), johar (Senna siamea), jintan hitam (Nigella sativa), apukat (Persea americana), dadap serep (Erythrina lithosprema), nagasari (Palaquium rostratum), pisang (Musa paradisiaca), sidaguri (Sida rhombifolia), tomat (Solanum lycopersicum), kethuk (Alocasia macrorrhiza) dan komak (Dolichos lablab). Penelitian ini bertujuan untuk membuktikan aktivitas antifungi ekstrak etanol tumbuhan uji dan menentukan nilai Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimumnya (KBM). Masing-masing simplisia tumbuhan uji diekstraksi dengan metode maserasi menggunakan pelarut etanol 70% selama 5 hari. Pengujian aktivitas antifungi terhadap Malassezia furfur menggunakan metode difusi sumuran, yang dilanjutkan dengan penentuan KHM dan KBM dengan metode dilusi. Hasil penelitian menunjukkan bahwa semua ekstrak etanol tumbuhan uji memiliki aktivitas antifungi yang bervariasi terhadap Malassezia furfur. Aktivitas yang kuat ditunjukkan oleh ekstrak etanol kulit buah pisang, daun bambu tali, daun komak, biji jintan hitam dan daun inggu. Nilai KHM masing-masing ekstrak antara lain: kulit buah pisang, daun bambu tali dan daun komak (10%), biji jintan hitam (20%), daun inggu (30%), daun dadap serep dan daun johar (50%), daun tomat (60%), daun apukat dan daun nagasari (70%). Ekstrak etanol daun sidaguri dan daun kethuk tidak memiliki nilai KHM. Semua ekstrak etanol tumbuhan uji tidak memiliki nilai Kadar Bunuh Minimum (KBM).
 Kata kunci: antifungi, ekstrak etanol, ketombe
 Abstract
 Dandruff is one of hair problem caused by Malassezia furfur, which can be controlled by antifungal agents. Empirically, some plants have been used as antidandruff treatment: rue (Ruta angustifolia), bamboo tali (Gigantochloa apus), blackwood cassia (Senna siamea), black cummin (Nigella sativa), avocado (Persea americana), Indian coral tree (Erythrina lithosprema), gutta percha (Palaquium rostratum), banana (Musa paradisiaca), cuban jute (Sida rhombifolia), tomato (Solanum lycopersicum), taro (Alocasia macrorrhiza) and lablab (Dolichos lablab). This research was aimed to observe antifungal activity, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of these 12 potential plants against Malassezia furfur. Each of plant simplisia was extracted by immersion in 70% ethanol  for 5 days. Antifungal activity assay against Malassezia furfur were conducted using agar well diffusion, followed by dilution method to determine MIC and MFC. The results showed that all of plant ethanolic extract have various antifungal activity against Malassezia furfur. Strong activity showed by banana peels extract, bamboo tali leaves extract, lablab leaves extract, black cumin seeds extract and rue leaves extract. MIC values obtained were 10 % for banana peels extract, bamboo tali and lablab leaves extract,  20% for black cumin seeds extract, 30% for rue leaves extract, 50% for Indian coral and blackwood cassia leaves extract, 60% for tomato leaves extract, 70% for avocado and gutta percha leaves extract. Whereas cuban jute and taro leaves extract did not show MIC values. All of plant ethanolic extracts did not show MFC values.
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 Key words: antifungal, dandruff, ethanolic extract
References
mid, A., S. Semail, P. Jamal. (2011). Tomato Leaves Methanol Extract Possesses Antiinflamatory Activity via Inhibition of Lipopolysacharide (LPS)-Induced Prostaglandin (PGE2). African Journal of Biotechnology Vol. 10 (81): 18674-18678
Arif, T., J. D. Bhosale, N. Kumar, T. K. Mandal, R. S. Bendre, G. S. Lavekar, R. Dabur. (2009). Natural Products–Antifungal Agents Derived from Plants. Journal of Asian Natural Products Research Vol. 11 No. 7: 621-638
Bukar, A., Mukhtar, M.D. and Hassan, A.S. (2009). Phytochemical Screening and Antibacterial Activity of Leaf Extracts of Senna siamea (Lam) on Pseudomonas aeruginosa. Bayero Journal of Pure and Applied Sciences, 2(1):139 - 142
Chhavi, S., D. Sushma, A. Mohammad. (2011). Potential of Herbal As Antidandruff Agents. International Journal Research of Pharmacy. Vol 2 (3): 16-18
Dhuhita, A. (2008). Aktivitas Minyak Atsiri Rimpang Bengle (Zingiber cassumunar roxb.) terhadap Pertumbuhan Malassezia furfur In Vitro. Artikel Karya Tuis Ilmiah. Universitas Diponegoro. Semarang
Freiesleben, S.H and A.K. Jager. (2014). Correlation between Plant Secondary Metabolites and Their Antifungal Mechanisms-A Review. Medicinal and Aromatic Plants Vol. 3 (2)
Haddouchi, F., T.M. Chaouche, Y. Zaouali, R. Ksouri, A. Attou, A. Benmansour. (2013). Chemical Composition and Antimicrobial Activity of The Essential Oils from Four Ruta Species Growing in Algeria. Food Chemistry 141: 253-258
Kobayashi, F., K. Ishida, H. Ikeura, S. Odake, Y. Hayata. (2012). Application of Tomato (Solanum lycopersicum) Leaf Volatiles as Antifungal Agents against Plant Pathogenis Fungi. Journal of Agricultural Sciences Vol. 4 No. 8: 231-235
Kumar, S. (2013). Analysis on the Natural Remedies to Cure Dandruff/Skin Disease-causing Fungus-Malassezia furfur. Advanced Bio Tech. ISSN: 2319-6750 Vol. 12 Issue 07: 01-05
Logeswari, P., V. Dineshkumar, S.M. Prathap Kumar, and P.T.A. Usha. (2013). In-Vivo Antiinflammatory Effect of Aqueous and Ethanolic Extract of Sida rhombifolia L. Root. International Journal of Pharmaceutical Science and Research Vol. 4(1): 316-321
Mita, S. R., D. Rusmiati, S. A. F. Kusuma. (2009). Pengembangan Ekstrak Etanol Kubis (Brassica oleracea var. Capitata L.) Asal Kabupaten Bandung Barat dalam Bentuk Sampo Antiketombe terhadap Jamur Malassezia furfur. Laporan Akhir Penelitian Peneliti Muda (LITMUD) tidak diterbitkan. Bandung: Universitas Padjadjaran
Mubeen, U. S., M. Vimlesh, B. Santanu. (2012). Laxative and Diuretic Property of Ethanolic Extract of Leaves of Allocasia macrorrhiza Linn. on Experimental Albino Rats. International Research journal of Pharmacy 3 (2): 174-176
Mulyono, N., B. W. Lay, L. Ocktreya, S. Rahayu. (2013). Antidiarrheal Activity of Apus Bamboo (Gigantochloa apus) Leaf Extract and Its Bioactive Compounds. American Journal of Microbiology 4 (1): 1-8
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