Determination of Total Phenolic, Flavonoid, and DPPH Scavenging Activity of Ruku-ruku (Ocimum tenuiflorum L.) Leaf

Haiyul Fadhli; Rizzati Islami; Rahayu Utami; Rahma Dona

doi:10.30595/pharmacy.v0i0.15200

Determination of Total Phenolic, Flavonoid, and DPPH Scavenging Activity of Ruku-ruku (Ocimum tenuiflorum L.) Leaf

Haiyul Fadhli, Rizzati Islami, Rahayu Utami, Rahma Dona

Abstract

Ruku-ruku (Ocimum tenuiflorum L.) is a plant that contains essential oils as well as phenolic and flavonoid compounds that act as antioxidants. This study aims to determine the total phenolic (TPC) and flavonoid content (TFC) and the DPPH scavenging activity of ruku-ruku leaves. Ruku-ruku leaf extraction was carried out using the maceration method using methanol. Measurement of TPC by Folin Ciocalteu method, TFC by AlCl₃ complex formation method, and antioxidant activity by DPPH method. The test results using a microplate reader showed that the methanol extract of ruku-ruku leaves had a total phenolic content of 300.71±2.86 mgGAE/g extract and total flavonoid content of 66.42±10.17 mgQE/g extract as well as very weak antioxidant activity (IC₅₀ = 2501.07±1.2 µg/mL).

Keywords

Antioxidant; Total Phenolic Content; Total Flavonoid Content; Ruku-Ruku (Ocimum tenuiflorum L.)

References

Aggarwal, A. & Mali, R.R. 2015. Ocimum tenuiflorum – A Medicinal Plants with its Versatile Uses. International Journal of Recent Advances in Science and Technology, 2(2): 1–10.

Alain, N. & Manullang, H.F. 2023. Antibakteri Ekstrak Etanol Daun Ruku-Ruku (Ocimum tenuiflorum L.) Terhadap Bakteri Staphylococcus epidermidis. BEST Journal (Biology Education, Sains and Technology), 6(2): 647–653.

Aleixandre-Tudo, J.L., Buica, A., Nieuwoudt, H., Aleixandre, J.L. & du Toit, W. 2017. Spectrophotometric Analysis of Phenolic Compounds in Grapes and Wines. Journal of agricultural and food chemistry, 65(20): 4009–4026.

Aminah, A., Tomayahu, N. & Abidin, Z. 2016. Penetapan Kadar Flavonoid Total Ekstrak Etanol Kulit Buah Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri Uv-Vis. Fitofarmaka Indonesia, 4(2): 226–230.

Asuero, A.G., Sayago, A. & González, A.G. 2006. The Correlation Coefficient: An Overview. Critical reviews in analytical chemistry, 36(1): 41–59.

Banjarnahor, S.D.S. & Artanti, N. 2014. Antioxidant Properties of Flavonoids. Medical Journal of Indonesia, 23(4): 239–244.

Barchan, A., Bakkali, M., Arakrak, A., Pagán, R. & Laglaoui, A. 2014. The Effects of Solvents Polarity on The Phenolic Contents and Antioxidant Activity of Three Mentha Species Extracts. Int. J. Curr. Microbiol. Appl. Sci, 3(11): 399–412.

Borges, L.L., Alves, S.F., Sampaio, B.L., Conceição, E.C., Bara, M.T.F. & Paula, J.R. 2013. Environmental Factors Affecting the Concentration of Phenolic Compounds in Myrcia tomentosa Leaves. Revista Brasileira de Farmacognosia, 23(2): 230–238.

Cicco, N., Lanorte, M.T., Paraggio, M., Viggiano, M. & Lattanzio, V. 2009. A Reproducible, Rapid and Inexpensive Folin–Ciocalteu Micro-method in Determining Phenolics of Plant Methanol Extracts. Microchemical journal, 91(1): 107–110.

Dabire, C., Nebie, R.H.C., Belanger, A., Nacro, M. & Sib, F.S. 2011. Effet du séchage de la matière végétale sur la composition chimique de l’huile essentielle et l’activité antioxydante d’extraits de Ocimum basilicum L. International Journal of Biological and Chemical Sciences, 5(3): 1082–1095.

Divisha, R., Ranganathan, V., Vijayakaran, K. & Elamaran, A. 2018. Evaluating Ocimum basilicum and Ocimum tenuiflorum leaf extracts for the presence of phenolic compounds. 7(6): 2453–2456.

Engwa, G.A. 2018. Free Radicals and The Role of Plant Phytochemicals as Antioxidants against Oxidative Stress-related Diseases. Phytochemicals: source of antioxidants and role in disease prevention. BoD–Books on Demand, 7: 49–74.

Erdityo, C.W., Tanjung, M.M.. & Tjahjandarie, T.S. 2014. Isolasi dan Identifikasi Senyawa Fenolik Dari Batang Bauhinia semibifida Serta Uji Aktivitas Antikanker Terhadap Sel Murin Leukemia P-388. ADLN - Perpustakaan Universitas Airlangga.

Fadhli, H., Islami, R., Asparyzha, R., Putri, H., Lutica, H., Patmawati, L.., Utami, R., Jumadilla, I.M. & Salsabila, O.M. 2022. Tumbuhan Berpotensi Obat Disekitar Kita : Ruku-ruku, Tumbuhan Kupu-kupu, Tuntun Angin, Derendan dan Turi. Pekanbaru: Sekolah Tinggi Ilmu Farmasi Riau.

Halbwirth, H. 2010. The Creation and Physiological Relevance of Divergent Hydroxylation Patterns in The Flavonoid Pathway. International journal of molecular sciences, 11(2): 595–621.

Harborne, J.. 2016. Metode Fitokimia: Penentuan Cara Modern Menganalisis Tumbuhan. 2 ed. Bandung: ITB.

Julianto, T.S. 2019. Fitokimia : Tinjauan Metabolit Sekunder dan Skrining Fitokimia. Yogyakarta: Universitas Islam Indonesia.

Kedare, S.B. & Singh, R.P. 2011. Genesis and Development of DPPH Method of Antioxidant Assay. Journal of Food Science and Technology, 48(4): 412–422.

Kelly, G.S. 2011. Quercitin. Alternative Medicine Review, 16(2): 172–194.

Khoddami, A., Wilkes, M.A. & Roberts, T.H. 2013. Techniques for Analysis of Plant Phenolic Compounds. Molecules, 18(2): 2328–2375.

Koleva, I.I., Van Beek, T.A., Linssen, J.P.H., Groot, A. de & Evstatieva, L.N. 2002. Screening of Plant Extracts for Antioxidant Activity: A Comparative Study on Three Testing Methods. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 13(1): 8–17.

Marinova, G. & Batchvarov, V. 2011. Evaluation of The Methods for Determination of The Free Radical Scavenging Activity by DPPH. Bulgarian Journal of Agricultural Science, 17(1): 11–24.

Marliana, S.D. & Suryanti, V. 2005. Skrining Fitokimia dan Analisis Kromatografi Lapis Lipis Komponen Kimia Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol. Biofarmasi, 3(1): 26–31.

Muchtadi, D. 2013. Antioksidan dan Kiat Sehat di Usia Produktif. Bandung: Alfabeta, 83.

Nayeem, N., Asdaq, S.M.B., Salem, H. & AHEl-Alfqy, S. 2016. Gallic Acid: A Promising Lead Molecule for Drug Development. Journal of Applied Pharmacy, 8(2): 1–4.

Rahayu, S., Kurniasih, N. & Amalia, V. 2015. Ekstraksi dan Identifikasi Senyawa Flavonoid dari Limbah Kulit Bawang Merah sebagai Antioksidan Alami. al Kimiya: Jurnal Ilmu Kimia dan Terapan, 2(1): 1–8.

Raina, A.P., Kumar, A. & Dutta, M. 2013. Chemical Characterization of Aroma Compounds in Essential Oil Isolated from “Holy Basil” (Ocimum tenuiflorum L.) Grown in India. Genetic Resources and Crop Evolution, 60(5): 1727–1735.

Rawel, H.M., Czajka, D., Rohn, S. & Kroll, J. 2002. Interactions of Different Phenolic Acids and Flavonoids with Soy Proteins. International Journal of Biological Macromolecules, 30(3–4): 137–150.

Salim, Z.& E.M. 2017. Info Komoditi Tanaman Obat. Jakarta: Badan Pengkajian dan Pengembangan Perdagangan.

Setha, B., Gaspersz, F.F., Idris, A.P.S., Rahman, S. & Mailoa, M.N. 2013. Potential of Seaweed Padina sp. as A Source of Antioxidant. International Journal of Scientific and Technology Research, 2(6): 221–224.

Sudarsono, P.N., Gunawan, D., Wahyuono, S. & Donatus, I.A. 2002. Tumbuhan Obat II (Hasil Penelitian, Sifat-sifat dan Penggunaan). Yogyakarta: Universitas Gadjah Mada.

Treml, J. & Šmejkal, K. 2016. Flavonoids as Potent Scavengers of Hydroxyl Radicals. Comprehensive reviews in food science and food safety, 15(4): 720–738.

Upadhyay, R., Nachiappan, G. & Mishra, H.N. 2015. Ultrasound-assisted Extraction of Flavonoids and Phenolic Compounds from Ocimum tenuiflorum Leaves. Food Science and Biotechnology, 24: 1951–1958.

Wahyuni, S., Hidayat, Y. & Vivi, F. 2017. Daya Hambat Ekstrak Bunga Ruku-Ruku (Ocimum sanctum L.) Terhadap Pertumbuhan Streptococcus mutans. Jurnal STKIP PGRI, 9(3): 237–241.

WHO 2019. WHO Global Report On Traditional and Complementary Medicine 2019. World Health Organization. Luxembourg: WHO publications.

Xu, B.J. & Chang, S.K.C. 2007. A Comparative Study on Phenolic Profiles and Antioxidant Activities of Legumes as Affected by Extraction Solvents. Journal of Food Science, 72(2): S159–S166.

Zuraida, Z., Sulistiyani, S., Sajuthi, D. & Suparto, I.H. 2017. Fenol, Flavonoid, dan Aktivitas Antioksidan pada Ekstrak Kulit Batang Pulai (Alstonia scholaris R. Br). Jurnal Penelitian Hasil Hutan, 35(3): 211–219.

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DOI: 10.30595/pharmacy.v0i0.15200

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