ANALISIS PENAMBATAN MOLEKUL SENYAWA FLAVONOID BUAH MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.) PADA RESEPTOR α-GLUKOSIDASE SEBAGAI ANTIDIABETES

Authors

  • Rizky Arcinthya Rachmania Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. Hamka Islamic Center, Jl. Delima II/IV, Klender Jakarta Timur 13460
  • Supandi Supandi Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. Hamka Islamic Center, Jl. Delima II/IV, Klender Jakarta Timur 13460
  • Frisca Ananda Dwi Cristina Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. Hamka Islamic Center, Jl. Delima II/IV, Klender Jakarta Timur 13460

Abstract

Mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl.) merupakan buah yang banyak mengandung flavonoid yang dapat digunakan sebagai obat antidiabetes. Senyawa flavonoid yang berkhasiat antidiabetes pada buah mahkota dewa belum diketahui jenisnya sehingga perlu diteliti. Penelitian ini bertujuan untuk melihat interaksi senyawa flavonoid buah mahkota dewa terhadap enzim α-glukosidase melalui nilai energi bebas (binding affinity/ΔG˚) dan untuk mendapatkan jenis flavonoid yang sesungguhnya dari buah mahota dewa sebagai tahap pencarian kandidat obat antidiabetes yang baru. Pelaksanaan penelitian ini diawali dengan mencari struktur dari enzim α-glukosidase di RCSB kemudian dilakukan proses penambatan molekul, diamati visualisasinya melalui Pymol dan penentuan pocket cavity. Hasil penambatan molekul terhadap 10 senyawa flavonoid menunjukkan senyawa yang memiliki energi bebas terendah adalah fevicordin A dengan nilai -10,8 kcal/mol, sementara itu hasil visualisasi dari fevicordin A terdapat 5 residu asam amino yaitu; Leu 286, Phe 535, Ile 523, Ser 521, Arg 520. Phaleria (Phaleria macrocarpa (Scheff.) Boerl.) is a fruit that contains flavonoids which can use as antidiabetic. Flavonoid compounds type in Phaleria is unknown, so it is need to be investigated. Purpose of this study were to see the interaction of flavonoids of Phaleria with α-glucosidase enzyme by binding affinity and to find out the most potent flavonoid of Phaleria as new drug candidate for antidiabetes. Implementation of this study begins with the search for the structure of the α-glucosidase enzyme in RCSB and then docking process, observed visually with Pymol and determination of pocket cavity. Results of molecular docking of 10 flavonoids, showed that the flavonoid with lowest free energy was fevicordin A with the value of 10.8 kcal/mol. The results visualization showed that fevicordin A have 5 amino acid residues.

References

Altaf, R., Zaini, M., Dewa, A. 2013. Phytochemistry and medicinal properties of Phaleria macrocarpa (Scheff.) Boerl extracts. Pharmacognosy Reviews, 7(13):73-80. Kemenkes. 2013. Riset Kesehatan Dasar: Riskesdas 2013. Jakarta: Badan Penelitian dan Pengembangan Kesehatan Kementrian Kesehatan RI. Data diakses melalui http://www.litbang.depkes.go.id/riskesnas pada 12 Desember 2014. Malviya, N., Jain, S., Malviya, S. 2010. Antidiabetic potential of medicinal plants. Acta Poloniae Pharmaceutica-Drug Research; 67(2): 113-118. Meiyanti, Dewoto, H.R., Suyatna, D.F. 2006. Efek hipoglikemik daging buah mahkota dewa (Phaleria Macrocarpa (Scheff.) Boerl. terhadap kadar gula darah pada manusia sehat setelah pembebanan glukosa. Universa Medicina, 25(3):114-120. Misnadiarly. 2006. Diabetes Mellitus: Gangren, Ulcer, Infeksi, Mengenal Gejala, Menanggulangi, dan Mencegah Komplikasi. Jakarta: Pustaka Populer Obor. Roy, D., Kumar, V., Thirumurugan, K., Acharya, K. 2013. Probing the binding of syzygium-derived α-glucosidase inhibitors with N- and C-terminal human maltase glucoamylase by penambatan molekul and molecular dynamics simulation. Applied Biochemistry and Biotechnology, 172(1):102-114. Suarsana, N., Prioseryanto, B.P., Bintang, M., Wresdiyati, T. 2008. Aktivitas daya hambat enzim α-glukosidase dan efek hipoglikemik ekstrak tempe pada tikus diabetes. Jurnal Veteriner, 9(3):122-127 Sugiwati, S., Efy, A., Setiasih, S. 2006. α-glucosidase inhibitory activity and hypoglycemic effect of Phaleria macrocarpa fruit pericarp extract by oral administration to rats. Journal of Appied Sciences, 6(10):2312-2316. Yanuar, A. 2012. Penambatan Molekular Praktek dan Aplikasi pada Virtual Screening. Depok: Laboratorium Komputasi Biomedik dan Rancangan Obat, Fakultas Farmasi Universitas Indonesia.

Downloads

Published

2016-12-01

How to Cite

Rachmania, R. A., Supandi, S., & Cristina, F. A. D. (2016). ANALISIS PENAMBATAN MOLEKUL SENYAWA FLAVONOID BUAH MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.) PADA RESEPTOR α-GLUKOSIDASE SEBAGAI ANTIDIABETES. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 13(02), 239–251. Retrieved from http://jurnalnasional.ump.ac.id/index.php/PHARMACY/article/view/1260

Issue

Jurnal Pharmacy, Vol. 13 No. 02 Desember 2016

Section

PHARMACY

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).