Pemisahan dan Pemurnian Senyawa Sulforaphane pada Brokoli (Brassica oleracea L.) dan Aktivitas Sitotoksik terhadap Sel Kanker T47D

Muladi Putra Mahardika; Azis Saifudin

doi:10.30595/pharmacy.v18i1.9295

Pemisahan dan Pemurnian Senyawa Sulforaphane pada Brokoli (Brassica oleracea L.) dan Aktivitas Sitotoksik terhadap Sel Kanker T47D

Muladi Putra Mahardika, Azis Saifudin

Abstract

Brokoli (Brassica oleracea L.) merupakan sayuran dalam keluarga Brassicaceae (Cruciferae) yang kaya akan senyawa bioaktif, salah satunya adalah sulforaphane. Penelitian menunjukan bahwa senyawa sulforaphane dalam brokoli memiliki potensi sebagai agen anti kanker. Metode isolasi dan analisa sulforaphane secara sederhana dan murah dibutuhkan untuk mendapatkan sulforaphane murni dengan mengoptimalkan jenis pelarut dan fase gerak dalam proses ekstraksi dan pemisahannya. Senyawa sulforaphane diperoleh melalui proses ekstraksi, fraksinasi dan purifikasi serta proses identifikasi menggunakan NMR dan FT-IR. Isolat hasil purifikasi diuji aktivitas antikanker dengan metode 3-(4,5-dimethyl thiazolyl-2 - yl)-2, 5- diphenyl tetrazolium bromide (MTT) terhadap sel T47D. Hasil penelitian menunjukan sulforaphane dapat di isolasi dan di analisis dengan FTIR serta H-NMR dengan metode ekstraksi yang sederhana. Hasil isolasi dan identifikasi senyawa diperoleh satu isolat, yaitu sulforaphane yang merupakan senyawa golongan isothiocyanate. Nilai IC₅₀ terhadap sel T47D dari ekstrak brokoli, fraksi etil esetat brokoli, dan isolat masing-masing sebesar 46.853,00; 25.559,00; dan 109.950,00 µg/ml.

Keywords

Brassica oleracea L.; MTT assay; senyawa aktif; sel T47D; sitotoksik; sulforaphane

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DOI: 10.30595/pharmacy.v18i1.9295

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