Identifikasi Senyawa Flavonoid Daun Jinten (Plectranthus amboinicus) Menggunakan Liquid Chromatography-Mass Spectrometry (LC-MS) dan Potensinya sebagai Antikanker Secara In-Vitro
Abstract
Penelitian ini mengungkapkan potensi senyawa flavonoid dalam daun jinten (Plectranthus amboinicus) sebagai agen antikanker. Melalui identifikasi menggunakan Liquid Chromatography-Mass Spectrometry (LC-MS), ditemukan delapan senyawa flavonoid, termasuk Kaempferol 3-(5''-feruloylapioside), Kaempferol 3-(6''-caffeoylglucoside), dan Kaempferol 3-glucosyl-(1 2)-galactosyl-(1 2)-glucoside. Senyawa-senyawa ini telah terbukti memiliki potensi antikanker melalui penelitian sebelumnya. Metode LC-MS dipilih untuk identifikasi senyawa karena memberikan data yang informatif, termasuk informasi tentang massa molekul senyawa yang teridentifikasi. Proses penelitian melibatkan beberapa langkah, mulai dari pembuatan simplisia daun jinten, pembuatan ekstrak melalui maserasi, fraksinasi, hingga identifikasi senyawa menggunakan LC-MS. Temuan ini mengindikasikan bahwa daun jinten memiliki potensi sebagai sumber senyawa antikanker yang bernilai, yang dapat menjadi dasar untuk penelitian lebih lanjut dalam memanfaatkannya dalam terapi kanker.
Keywords
References
Agati, G., Biricolti, S., Guidi, L., Ferrini, F., & Fini, A. (2021). Flavonoids as antioxidants in plants under abiotic stresses. International Journal of Molecular Sciences, 22(7), 3520.
Araújo, J. R., Gonçalves, P., & Martel, F. (2011). Chemopreventive effect of dietary polyphenols in colorectal cancer cell lines. Nutrition Research, 31(2), 77–87. doi: 10.1016/j.nutres.2011.01.006.
Chao J, Dai Y, Verpoorte R, Lam W, Cheng YC, Pao LH, Hsu JL, Chao LK. Anti-cancer effects of emodin on HepG2 cells as revealed by 1H-NMR based metabolic profiling. J Pharm Biomed Anal. 2014;90:160-165.
Falcone Ferreyra, M. L., Casas, M. I., Questa, J. I., Herrera, A. L., Deblasio, S., Wang, J., ... & Casati, P. (2020). Evolution and functional diversification of flavonoid biosynthesis in terrestrial plants. The Plant Journal, 101(5), 756-778.
Guo, R., Guo, H., Hao, W., Yao, P., & Chen, L. (2020). Kaempferol exhibits anti-inflammatory effects in LPS-stimulated RAW264. 7 cells and inflammation-induced mice by inflammasome activation and pyroptosis signaling pathway. Inflammation, 43(3), 776-789.
Gomathi, D., Kalaiselvi, M., & Ravikumar, G. (2019). Phytochemical composition and antimicrobial activity of essential oil from Plectranthus amboinicus (Lour.) Spreng. International Journal of Green Pharmacy, 13(1), 61-66.
Imran M, Salehi B, Sharifi-Rad J, Aslam Gondal T, Saeed F, Imran A, Shahbaz M, Tsouh Fokou PV, Umair Arshad M, Khan H, Guerreiro SG, Martins N, Estevinho LM. Kaempferol: A Key Emphasis to Its Anticancer Potential. Molecules. 2019; 24(12):2277.
Kim YH, Woo H, Kim JS, Shin KO, Lee JH, Jang DS, Choi JH. Kaempferol 3-glucosyl-(1-->2)-galactosyl-(1-->2)-glucoside from the leaves of Ailanthus altissima. J Korean Soc Appl Biol Chem. 2013;56(6):731-737.
Liu L, Su X, Zhang X, Qi J, Yang Y, Zhou Q. Antiproliferative and proapoptotic activities of quercetin-3-O-(6''-feruloyl)-beta-D-glucopyranoside in human breast cancer cells. Nutr Cancer. 2012;64(8):1228-1236.
Liao Y, Shen W, Kong G, Lv H, Tao W, Bo P, et al. Antitumor activities of quercetin and quercetin-5',8-disulfonate in human colon and breast cancer-derived cells. Tumour Biol. 2016;37(6):7939-7949.
Lee JK, Cho YS, Park K. Enhancement of antioxidant and anticancer activities of soybean koji fermented with Bacillus amyloliquefaciens. Food Sci Biotechnol. 2017;26(6):1699-1706.
Lee J, Park SY, Kim YS, Kim YH. Quercetin 3-(3'',6''-di-p-coumarylglucoside) induces apoptosis in human breast cancer cells by inhibiting EGFR signaling. Free Radic Biol Med. 2017;112:228-236.
Lee SJ, Kim JY, Kang DG, Lee HS. Kaempferol 3-O-(5"-feruloylapioside) isolated from Perilla frutescens suppresses invasion and MMP-9 expression of HT1080 cells. Nat Prod Sci. 2012;18(1):29-34.
Ravishankar, D., Rajora, A. K., Greco, F., Osborn, H. M., & Flavonoids as prospective compounds for anti-cancer therapy. The International Journal of Biochemistry & Cell Biology, 89, 232-246.
Olivares, M., Pourmasoumi, M., Ruiz, F. G., Câmara, J. S., Trindade, R. C., & Lorenzo, J. M. (2019). Liquid chromatography–mass spectrometry: A versatile tool in food chemistry. Food Research International, 115, 23-63.
Park, J. Y., Han, X. H., & Ko, E. J. (2018). Anti-inflammatory effects of Plectranthus amboinicus (Lour.) Spreng (Mexican mint) essential oil in vitro and in vivo models. Journal of Ethnopharmacology, 227, 155-162.
Salehi, B., Venditti, A., Sharifi-Rad, M., Kręgiel, D., Sharifi-Rad, J., Durazzo, A., ... & Zucca, P. (2019). The therapeutic potential of apigenin. International Journal of Molecular Sciences, 20(6), 1305.
Sghaier, M. B., Pagano, A., Mousslim, M., Ammari, Y., Kovacic, H. & Luis, J. (2016). Rutin Inhibits Proliferation, Attenuates Superoxide Production and Decreases Adhesion and Migration of Human Cancerous Cells. Biomedicine & Pharmacotheraphy, 84, 1972–1978.
Singh, A. P., De, P., Singh, R., Mishra, A., & Jaiswal, A. (2022). Neuroprotective potential of Plectranthus amboinicus (Lour.) Spreng against rotenone-induced neurotoxicity in rat model of Parkinson's disease. Journal of Ethnopharmacology, 282, 114579.
Tatiya, A. U., Saluja, A. K., & Surana, S. J. (2021). Evaluation of antihyperglycemic activity of Plectranthus amboinicus in normal and streptozotocin-induced diabetic rats. International Journal of Pharmaceutical Sciences and Research, 12(1), 524-529.
Xie, X. Y., Liu, J. Y., Hao, Q., & Yang, C. Q. (2021). Flavonoids in plant–rhizobium interactions: potential roles and molecular mechanisms. Plant Molecular Biology, 105(1-2), 163-177.
Yuliana, N. D., Iqbal, M., Susilowati, R., & Darwati, I. (2020). Evaluation of antimicrobial activity of Plectranthus amboinicus Lour. extracts against Streptococcus mutans. Journal of International Dental and Medical Research, 13(4), 1340-1344.
Yang X, Li X, Ren X, Meng X, Pang J, Wen L. Antitumor activities of quercetin and quercetin-5',8-disulfonate in human colon and breast cancer cell lines. Food Chem Toxicol. 2014;70:151-161.
Yao F, Wang P, Luo J, Zhang G. Cytotoxic and apoptotic effects of kaempferol 3-O-(6''-O-E-caffeoyl-glucoside) on the hepatocellular carcinoma cell line HepG2. Biomed Pharmacother. 2016;81:64-71
Yang F, Song L, Wang H, Wang J, Xu Z, Xing N. Quercetin in prostate cancer: Chemotherapeutic and chemopreventive effects, mechanisms and clinical application potential (Review). Oncol Rep. 2015; 33(6):2659-68)
Wu Y, Zhang H, Dong Y, et al. Rutin improves spatial memory in Alzheimer's disease transgenic mice by reducing Aβ oligomer level and attenuating oxidative stress and neuroinflammation. Behav Brain Res. 2016;296:296-304.
Zhang L, Liu Y, Lu XT, Xu J, Ding K. Kaempferol 3-glucosyl-(1-2)-galactosyl-(1-2)-glucoside inhibits invasion of hepatocellular carcinoma via down-regulation of matrix metalloproteinases-2 and -9. Phytother Res. 2014;28(1):69-75.
DOI: 10.30595/sainteks.v21i1.21171
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN: 2686-0546
Reference Tools
Abstracting & Indexing
Email this article (Login required)
Email the author (Login required)
