Antimicrobial activity of Etlingera elatior leaf extract in Staphylococcus aureus-induced cystitis: an in vivo study
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Background: Etlingera elatior has been reported to possess antimicrobial properties; however, its therapeutic effects in bacterial cystitis, particularly those caused by Staphylococcus aureus (S. aureus), remain inadequately explored in vivo.
Objective: This study aimed to evaluate the antimicrobial and anti-inflammatory effects of Etlingera elatior leaf extract in an experimental model of S. aureus cystitis.
Methods: A true experimental posttest-only design was conducted using 28 male BALB/c mice induced with S. aureus. The animals were allocated into control and treatment groups receiving Etlingera elatior leaf extract at concentrations of 40%, 50%, 60%, 70%, and 80%. Therapeutic outcomes were assessed based on urinary tract mucosal fluid secretion and bacterial colony counts. Data were analyzed using one-way ANOVA followed by post hoc testing.
Results: Treatment with Etlingera elatior leaf extract significantly reduced cystitis severity compared with the infected control group (p < 0.05). The extract demonstrated both antimicrobial and anti-inflammatory effects, as indicated by reductions in bacterial load and mucosal secretion. Among the tested concentrations, the 40% extract showed the most consistent therapeutic effect, with outcomes comparable to ciprofloxacin.
Conclusions: Etlingera elatior leaf extract exhibits potential as an alternative therapeutic agent for bacterial cystitis. Its antimicrobial and anti-inflammatory properties support its further development as a complementary treatment for urinary tract infections.
1. Mansfield KJ, Chen Z, Moore KH, Grundy L. Urinary Tract Infection in Overactive Bladder: An Update on Pathophysiological Mechanisms. Front Physiol. 2022;13(July):1-12. doi:10.3389/fphys.2022.886782
2. Tuon FF, Suss PH, Telles JP, Dantas LR, Borges NH, Ribeiro VST. Antimicrobial Treatment of Staphylococcus aureus Biofilms. Antibiotics. 2023;12(1):1-24. doi:10.3390/antibiotics12010087
3. Noordin L, Ahmad WANW, Nor NAM, Bakar NHA, Ugusman A. Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes. Evidence-based Complement Altern Med. 2022;2022. doi:10.1155/2022/2814196
4. Takó M, Kerekes EB, Zambrano C, et al. Plant phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms. Antioxidants. 2020;9(2). doi:10.3390/antiox9020165
5. Ecevit K, Barros AA, Silva JM, Reis RL. Preventing Microbial Infections with Natural Phenolic Compounds. Futur Pharmacol. 2022;2(4):460-498. doi:10.3390/futurepharmacol2040030
6. Juwita T, Puspitasari IM, Levita J. Torch ginger (Etlingera elatior): A review on its botanical aspects, phytoconstituents and pharmacological activities. Pakistan J Biol Sci. 2018;21(4):151-165. doi:10.3923/PJBS.2018.151.165
7. Mitropoulou G, Stavropoulou E, Vaou N, et al. Insights into Antimicrobial and Anti-Inflammatory Applications of Plant Bioactive Compounds. Microorganisms. 2023;11(5):1-15. doi:10.3390/microorganisms11051156
8. Roy A, Khan A, Ahmad I, et al. Flavonoids a Bioactive Compound from Medicinal Plants and Its Therapeutic Applications. Biomed Res Int. 2022;2022. doi:10.1155/2022/5445291
9. Yeshi K, Crayn D, Ritmejeryte E, Wangchuk P. Plant secondary metabolites produced in response to abiotic product development. Molecules. 2022;27(1):313.
10. Nor NAM, Noordin L, Bakar NHA, Ahmad WANW. Evaluation of antidiabetic activities of Etlingera elatior flower aqueous extract in vitro and in vivo. J Appl Pharm Sci. 2020;10(8):43-51. doi:10.7324/JAPS.2020.10805
11. Ricciotti E, Fitzgerald GA. Prostaglandins and inflammation. Arterioscler Thromb Vasc Biol. 2011;31(5):986-1000. doi:10.1161/ATVBAHA.110.207449
12. Chen L, Deng H, Cui H, et al. Oncotarget 7204 www.impactjournals.com/oncotarget Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9(6):7204-7218. www.impactjournals.com/oncotarget/
13. Sharma S, Mohler J, Mahajan SD, Schwartz SA, Bruggemann L, Aalinkeel R. Microbial Biofilm: A Review on Formation, Infection, Antibiotic Resistance, Control Measures, and Innovative Treatment. Vol 11.; 2023. doi:10.3390/microorganisms11061614
14. Ullah A, Munir S, Badshah SL, et al. Important Flavonoids and Their Role as a Therapeutic Therapeutic Agent. :1-39.
15. Ysrafil Y, Sapiun Z, Slamet NS, et al. Anti-inflammatory activities of flavonoid derivates. ADMET DMPK. 2023;11(3):331-359. doi:10.5599/admet.1918
16. Ribeiro VP, Arruda C, El-Salam MA, Bastos JK. Brazilian medicinal plants with corroborated anti-inflammatory activities: A review. Pharm Biol. 2018;56(1):253-268. doi:10.1080/13880209.2018.1454480
17. Graßmann J. Terpenoids as Plant Antioxidants. Vitam Horm. 2005;72(05):505-535. doi:10.1016/S0083-6729(05)72015-X
18. Del Prado-Audelo ML, Cortés H, Caballero-Florán IH, et al. Therapeutic Applications of Terpenes on Inflammatory Diseases. Front Pharmacol. 2021;12(August):1-7. doi:10.3389/fphar.2021.704197
19. Câmara JS, Perestrelo R, Ferreira R, Berenguer C V., Pereira JAM, Castilho PC. Plant-Derived Terpenoids: A Plethora of Bioactive Compounds with Several Health Functions and Industrial Applications—A Comprehensive Overview. Molecules. 2024;29(16). doi:10.3390/molecules29163861
20. Zhao M, Wu F, Tang Z, et al. Anti-inflammatory and antioxidant activity of ursolic acid: a systematic review and meta-analysis. Front Pharmacol. 2023;14(September):1-14. doi:10.3389/fphar.2023.1256946
21. Ernilasari, Walil K, Fitmawati, et al. Antibacterial activity of leaves, flowers, and fruits extract of etlingera elatior from nagan raya district, indonesia against escherichia coli and staphylococcus aureus. Biodiversitas. 2021;22(10):4457-4464. doi:10.13057/biodiv/d221039
22. Yan Y, Xia X, Fatima A, et al. Antibacterial Activity and Mechanisms of Plant Flavonoids against Gram-Negative Bacteria Based on the Antibacterial Statistical Model. Pharmaceuticals. 2024;17(3). doi:10.3390/ph17030292
23. Farha AK, Yang QQ, Kim G, et al. Tannins as an alternative to antibiotics. Food Biosci. 2020;38(January):100751. doi:10.1016/j.fbio.2020.100751
24. Huang Q, Liu X, Zhao G, Hu T, Wang Y. Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production. Anim Nutr. 2018;4(2):137-150. doi:10.1016/j.aninu.2017.09.004
25. Shamsudin NF, Ahmed QU, Mahmood S, et al. Antibacterial Effects of Flavonoids and Their Structure-Activity Relationship Study: A Comparative Interpretation. Molecules. 2022;27(4). doi:10.3390/molecules27041149
26. Zhang QY, Yan Z Bin, Meng YM, et al. Antimicrobial peptides: mechanism of action, activity and clinical potential. Mil Med Res. 2021;8(1):1-25. doi:10.1186/s40779-021-00343-2
27. Nono RN, Barboni L, Teponno RB, et al. Antimicrobial, antioxidant, anti-inflammatory activities and phytoconstituents of extracts from the roots of Dissotis thollonii Cogn. (Melastomataceae). South African J Bot. 2014;93:19-26. doi:10.1016/j.sajb.2014.03.009
28. Pizzino G, Irrera N, Cucinotta M, et al. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017;2017. doi:10.1155/2017/8416763
29. Chen Y, McMillan-Ward E, Kong J, Israels SJ, Gibson SB. Oxidative stress induces autophagic cell death independent of apoptosis in transformed and cancer cells. Cell Death Differ. 2008;15(1):171-182. doi:10.1038/sj.cdd.4402233
30. Goel N, Fatima SW, Kumar S, Sinha R, Khare SK. Antimicrobial resistance in biofilms: Exploring marine actinobacteria as a potential source of antibiotics and biofilm inhibitors. Biotechnol Reports. 2021;30:e00613. doi:10.1016/j.btre.2021.e00613
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