Uji Efektivitas Sedatif-Hipnotik Ekstrak Daun Keji Beling (Strobilanthes crispa Blume) terhadap Mencit Putih Jantan
DOI:
https://doi.org/10.30595/pharmacy.v0i0.14234Keywords:
Fireplace test, Insomnia, Sedatif-hipnotik, Strobilanthes crispaAbstract
Insomnia merupakan sebuah gangguan tidur yang bisa menimbulkan banyak masalah kesehatan, seperti menurunnya sistem kekebalan tubuh serta tekanan darah tinggi, sehingga perlu penanganan yang serius. Penanganan insomnia biasanya dilakukan dengan cara pemberian golongan obat sedatif-hipnotik seperti benzodiazepin dan barbiturat. Namun, golongan obat tersebut mempunyai efek samping yang berbahaya, mulai dari mual, sakit kepala ringan hingga vertigo. Penelitian tentang obat insomnia dari bahan alam telah banyak dilakukan. Salah satunya yaitu mengenai potensi keji beling (Strobilanthes crispa Blume) terhadap sedatif-hipnotik yang dilakukan secara in silico menggunakan metode molecular docking dan membuktikan bahwa kandungan d-limonen pada daun keji beling memiliki hasil yang paling baik terhadap aktivitas sedatif-hipnotik. Penelitian lanjutan ini bertujuan untuk mengetahui keefektifan ekstrak daun keji beling terhadap efek sedatif-hipnotik pada mencit. Metode yang digunakan adalah fireplace test, dengan parameter pengujian berupa lamanya waktu mencit untuk keluar dari dalam tabung yang diletakkan di atas penangas, hal ini untuk mengetahui berapa lama penurunan aktivitas gerak pada mencit. Objek penelitian sebanyak 15 ekor mencit jantan dibagi menjadi 5 kelompok yaitu, kontrol negatif (akuades), kontrol positif (diazepam 2 mg/70kgBB), perlakuan dosis 1 (200 mg/kgBB), dosis 2 (400 mg/kgBB), dan dosis 3 (600 mg/kgBB). Hasil penelitian menunjukkan bahwa rata-rata waktu mencit keluar dari tabung pada masing-masing kelompok perlakuan berturut-turut adalah 5, 120, 87, 118, dan 123 detik. Dari hasil penelitian ini, dosis III mempunyai efek sedatif-hipnotik yang lebih baik dari kontrol positif. Dari data yang didapatkan serta analisis ANOVA yang sudah dilakukan, dapat disimpulkan bahwa dosis 600 mg/kgBB memiliki efek yang lebih baik jika dibandingkan dengan kontrol positif yaitu diazepam.
References
Alnamer, R., Alaoui, K., Bouidida, E. H., Benjouad, A., & Cherrah, Y. 2012. Sedative and hypnotic activities of the methanolic and aqueous extracts of Lavandula officinalis from Morocco. Advances in Pharmacological Sciences. 2012: 270824. doi: 10.1155/2012/270824
Al-Henhena, N., Khalifa, S. A. M., Ying, R. P. Y., Ismail, S., Hamadi, R., Shawter, A. N., Idris, A. M., Azizan, A., Al-Wajeeh, N. S., Abdulla, M. A., & El-Seedi, H. R. 2015. Evaluation of chemopreventive potential of Strobilanthes crispus against colon cancer formation in vitro and in vivo. BMC Complementary and Alternative Medicine. 15(1): 419. doi: 10.1186/s12906-015-0926-7
Amir, N. 2007. Gangguan tidur pada lanjut usia - Diagnosis dan penatalaksanaan. Cermin Dunia Kedokteran.
Asthary, A.A., Yuharmen., Teruna, H.Y. 2014. Isolasi dan uji toksisitas senyawa metabolit sekunder dari ekstrak n-heksana daun tumbuhan Polyalthia rumphii (B) Merr. (Annonaceae). Repositori Universitas Riau.
Astuti, M.D., Umaningrum, D., Mustikasari, K. 2014. Toksisitas ekstrak n-heksana dan metanol daun kelompok tambahan tumbuhan permot (Passiflora foetida L). Jurnal Sains dan Terapan Kimia. 8(2): 80-86.
Begum, A., Hossen, A., Moly, A. A., & Bhuiyan, M. R. 2019. In vivo sedative and anxiolytic activities of Thunbergia erecta (Acanthaceae) leaves activate gamma-aminobutyric acid (GABA) mediated hyperpolarizationin Swiss albino mice. Pharmacology and Pharmacy. 10(4): 177–193. https://doi.org/10.4236/pp.2019.104016
Berkley, A. S., Carter, P. A., Yoder, L. H., Acton, G., & Holahan, C. K. 2020. The effects of insomnia on older adults’ quality of life and daily functioning: A mixed-methods study. Geriatric Nursing. 41(6):832-838. doi: 10.1016/j.gerinurse.2020.05.008.
Chang, W. P., & Lin, C. C. 2017. Relationships of salivary cortisol and melatonin rhythms to sleep quality, emotion, and fatigue levels in patients with newly diagnosed lung cancer. European Journal of Oncology Nursing. 29: 79-84. doi: 10.1016/j.ejon.2017.05.008.
Cheong, B. E., Zakaria, A., Ying, A., Cheng, F., Peik, & Teoh, L. 2016. GC-MS analysis of Strobilanthes crispus plants and callus. Transactions on Science and Technology, 3(1-2): 155 - 161.
Chua, L. Y. W., Chua, B. L., Figiel, A., Chong, C. H., Wojdyło, A., Szumny, A., & Choong, T. S. Y. 2019. Antioxidant activity, volatile and phytosterol contents of Strobilanthes crispus dehydrated using conventional and vacum microwave drying methods. Molecules. 24(7): 1397. doi: 10.3390/molecules24071397.
Ghasemzadeh, A., Jaafar, H. Z. E., & Rahmat, A. 2015. Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia. BMC Complementary and Alternative Medicine. 15(1):422. doi: 10.1186/s12906-015-0873-3.
Guo, J., Lou, M. P., Hu, L. L., & Zhang, X. 2020. Uncovering the pharmacological mechanism of the effects of the Banxia-Xiakucao Chinese herb pair on sleep disorder by a systems pharmacology approach. Scientific Reports. 10(1):20454. doi: 10.1038/s41598-020-77431-1.
Haida, Z., Nakasha, J., & Hakiman, M. 2020. Phenolics content and anioxidant properties of Strobilanthes crispus as affected by different extraction solvents. Fundamental and Applied Agriculture, 5(4): 584-589.
Harborne, J. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Cetakan kedua. Penerjemah: Padmawinata, K. dan I. Soediro. Bandung: Penerbit ITB.
Inayah, S., Gita, N.F., Bagas, K.J., Rizandi, J. 2021. Potensi daun keji beling (Strobilanthes crispus L.) sebagai kandidat obat untuk penderita insomnia melalui molecular docking senyawa sedatif hipnotik. Laporan Akhir: PKM RE. Sukabumi: Universitas Muhammadiyah Sukabumi.
Koay, Y. C., Wong, K. C., Osman, H., Eldeen, I. M. S., & Asmawi, M. Z. 2013. Chemical constituents and biological activities of Strobilanthes crispus L. Records of Natural Products, 7(1): 59-64.
Korpi, E. R., Mattila, M. J., Wisden, W., & Luddens, H. 2007. GABA(A)-receptor subtypes: Clinical efficacy and selectivity of benzodiazepine site ligands. Annals of Medicine. 29(4):275-82. doi: 10.3109/07853899708999348.
Lim, K. T., Lim, V., & Chin, J. H. 2012. Subacute oral toxicity study of ethanolic leaves extracts of Strobilanthes crispus in rats. Asian Pacific Journal of Tropical Biomedicine. 2(12): 948–952. doi: 10.1016/S2221-1691(13)60005-2.
Lin, Y. C., Sun, C. A., Lin, H. T., Perng, H. J., Chung, C. H., Lin, C. S., Lai, C. H., Chien, W. C., & Tsai, C. S. 2021. Increased risk of road traffic injuries in individuals with insomnia. Journal of Transport & Health. 21: 101030. doi: 10.1016/j.jth.2021.101030.
Liza, M. S., Abdul Rahman, R., Mandana, B., Jinap, S., Rahmat, A., Zaidul, I. S. M., & Hamid, A. 2012. Supercritical fluid extraction of bioactive flavonoid from Strobilanthes crispus (Pecah Kaca) and its comparison with solvent extraction. International Food Research Journal. 19 (2): 503-508.
Mahmud, S., Hossain, S., Muyeed, A., Islam, M. M., & Mohsin, M. 2021. The global prevalence of depression, anxiety, stress, and, insomnia and its changes among health professionals during COVID-19 pandemic: A rapid systematic review and meta-analysis. Heliyon. 7(7): e07393. doi: 10.1016/j.heliyon.2021.e07393.
Nugroho, R. 2018. Mengenal Mencit sebagai Hewan Laboratorium. Samarinda: Mulawarman University Pres.
Romadanu., Rachmawati, S.H., Lestari, S.D. 2014. Pengujian aktivitas antioksidan ekstrak bunga lotus (Nelumbo nucifera). Jurnal Fishtech. 3(1): 1-7. doi: https://doi.org/10.36706/fishtech.v3i1.3523
Seljeset, S., Bright D. P., Thomas P, Smart, T. G. 2018. Probing GABA A receptors with inhibitory neurosteroids. Neuropharmacology. 36: 23-36. doi: 10.1016/j.neuropharm.2018.02.008.
Sulastri, L., Lestari, R. M., & Simanjuntak, P. 2021. Isolasi dan identifikasi senyawa kimia monoterpen dari fraksi etil asetat daun keji beling (Strobilanthes crispa (L.) Blume) yang mempunyai daya sitotoksik. Jurnal Fitofarmaka Indonesia, 8 (1): 12-17.
Torres, V. I., & Weiss, D. S. 2002. Identification of a tyrosine in the agonist binding site of the homomeric ρ1 γ-aminobutyric acid (GABA) receptor that, when mutated, produces spontaneous opening. Journal of Biological Chemistry, 277(46), 43741–43748.https://doi.org/10.1074/jbc.M202007200.
Xu, F., Wang, X., Yang, Y., Zhang, K., Shi, Y., Xia, L., Hu, X., & Liu, H. 2021. Depression and insomnia in COVID-19 survivors: A cross-sectional survey from Chinese rehabilitation centers in Anhui province. Sleep Medicine. 91:161-165. doi: 10.1016/j.sleep.2021.02.002.
Yaacob, N. S., Yankuzo, H. M., Devaraj, S., Wong, J. K. M., & Lai, C. S. 2015. Anti-tumor action, clinical biochemistry profile and phytochemical constituents of a pharmacologically active fraction of S. crispus in NMU-induced rat mammary tumour model. PLoS ONE. 10 (5): 1-20.
Zhong, Y., Zheng, Q., Hu, P., Huang, X., Yang, M., Ren, G., Du, Q., Luo, J., Zhang, K., Li, J., Wu, H., Guo, Y., & Liu, S. 2019. Sedative and hypnotic effects of compound Anshen essential oil inhalation for insomnia. BMC Complementary and Alternative Medicine. 19(1), 1–11.
Zick, S. M., Wright, B. D., Sen, A., & Arnedt, J. T. 2011. Preliminary examination of the efficacy and safety of a standardized chamomile extract for chronic primary insomnia: A randomized placebo- controlled pilot study. BMC Complementary and Alternative Medicine. 11: 78. doi: 10.1186/1472-6882-11-78.
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