Uji Iritasi dan Uji Sifat Fisik Sediaan Gel Minyak Atsiri Daun Cengkeh (Syzygium aromaticum (L.) Merr. & L.M.Perry) dengan Variasi Konsentrasi HPMC
DOI:
https://doi.org/10.30595/pharmacy.v17i1.6528Keywords:
gel, HPMC, minyak atsiri daun cengkeh, uji iritasiAbstract
Inflamasi merupakan respon protektif normal terhadap luka jaringan yang disebabkan oleh trauma fisik, zat kimia yang merusak, atau zat-zat mikrobiologi. Salah satu bahan alam yang memiliki khasiat antiinflamasi adalah minyak atsiri daun cengkeh (Syzygium aromaticum (L.) Merr. & L.M.Perry). Penelitian ini bertujuan untuk mengetahui kualitas minyak atsiri daun cengkeh, formulasi gel minyak atsiri, serta uji sifat fisik dan uji iritasinya. Minyak atsiri diperoleh dengan destilasi uap dan air. Minyak atsiri yang didapat digunakan dalam sediaan gel dengan konsentrasi hidroksipropil metilselulose (HPMC) 3% (FI), HPMC 3% dan  minyak atsiri 6% (FII), HPMC 6% (FIII), HPMC 6% dan minyak atsiri 6% (FIV), HPMC 10% (FV), serta HPMC 10% dan minyak atsiri 6% (FVI). Pengamatan terhadap gel meliputi uji organoleptik, homogenitas, pH, uji daya lekat, dan uji daya sebar. Gel yang telah diuji sifat fisiknya, dievaluasi iritasinya terhadap kulit dengan metode Draize test. Data hasil pengujian dianalisis statistik dengan ANAVA satu arah dengan taraf kepercayaan 95%. Dari penelitian yang telah dilakukan diperoleh hasil minyak atsiri daun cengkeh yaitu berwarna kuning kecoklatan dan berbau khas daun cengkeh dengan nilai rendemen sebesar 1,6%, indeks bias sebesar 1,525, dan bobot jenis sebesar 1,02. Gel daun cengkeh memenuhi standar kualitas gel yaitu homogenitas, daya sebar,  pH, dan daya lekat. Analisis statistik terhadap masing-masing percobaan tidak menunjukkan perbedaan yang signifikan. Hasil percobaan terhadap uji iritasi menunjukkan bahwa sediaan gel tidak menimbulkan iritasi pada kulit marmut.
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