Effectiveness of Papaya (Carica papaya L.) Seed Extract and Alum on Aedes aegypti Larvae Death
DOI:
https://doi.org/10.30595/pharmacy.v21i1.14358Keywords:
Aedes aegypti, Alum, Larvicide, Micropartikel, Papaya Seeds, Spray DryAbstract
People generally use larvicide to kill mosquito larvae. Synthetic chemical larvicides leave residues that have a negative impact on the environment. Therefore, research on natural larvicides has been developed to suppress the negative effects of chemical larvicides. Microparticle preparations can speed up the compound delivery system so that it will be effective in its use as a larvicide. The important composition in making microparticle preparations is polymer. The polymer used in this research is chitosan. The formulation of papaya seed extract and alum microparticles using chitosan polymer with the spray drying method was made into three formulas with different concentrations of extract and alum, namely formula 1 (0.1% : 0.05%), formula 2 (0.15% : 0.075%). %), and formula 3 (0.2% : 0.1%). The results obtained show that formula 3 with a concentration of 0.2% papaya seed extract and 0.1% alum has the best quality characteristics based on the polydispersity index parameters, namely 0.181, particle size 1233.67 nm, zeta potential value 72.6 mV. The number of samples was 500 test larvae consisting of 5 treatment groups. Each group contained 25 larvae and 4 replicates. The test larvae were observed after 24 hours and then analyzed. The test analysis carried out was the Probit test. The LC50 value obtained in this study was 95.5 ppm. These results indicate that the microparticle powder preparation has larvicidal efficacy against third/IV instar Aedes aegypti larvae.
References
Agnihotri SA, Mallikarjuna NN, Aminabhavi TM. 2004. Recent advances on chitosan-based micro- and nanoparticles in drug delivery. Journal of Controlled Release, 100(1), 5-28.
Arimaswati, Sawaluddin LOM, Sudrajat HW. 2017. Efek larvasida ekstrak biji buah pepaya (Carica papaya L.) terhadap larva instar III Aedes aegypti L. Medula, 4(2), 332–343.
Asmira U, Sulasmi. 2019. Efektivitas serbuk biji pepaya (Carica papaya) dan tawas dalam mengendalikan jentik nyamuk Aedes aegypti. Jurnal Sulolipu: Media Komunikasi Sivitas Akademika dan Masyarakat, 19(1), 28-33.
Atmosoehardjo, S. 1991. Suatu Upaya Pengendalian Penggunaan Pestisida Melalui pendekatan Ilmu pengetahuan dan Teknologi. Surabaya: FK Unair.
BPOM RI. 2019. Peraturan Badan Pengawas Obat Dan Makanan tentang Persyaratan Keamanan Dan Mutu Obat Tradisional. Jakarta.
Dewantari KT, Yuliani S, Yasni S. 2013. Ekstraksi dan karakteristik nanopartikel ekstrak sirih merah (Piper crocatum), Jurnal Pasca Panen. 10(2), 65-71.
Husna, M., Dewi, R. S., dan Mirsiyanto, E. 2020. Efektivitas ekstrak bunga melati (Jasminum sambac L.) terhadap kematian larva Aedes aegypty. Journal of Healthcare Technology and Medicine, 6(2), 817-825.
Iskandar, I., Horiza, H., dan Fauzi, N. 2017. Efektivitas bubuk biji pepaya (Carica papaya Linnaeaus) sebagai larvasida alami terhadap kematian larva Aedes aegypty tahun 2015. Eksakta, 18(1), 12-18.
Jayanudin, Rochmadi, Renaldi MK, Pangihutan. 2017. Pengaruh bahan penyalut terhadap efisiensi enkapsulasi oleoresin jahe merah. ALCHEMY Jurnal Penelitian Kimia, 13(2), 275-287.
Karyanti, Rahma M, Hadinegoro SR. 2016. Perubahan epidemiologi demam berdarah dengue di Indonesia. Sari Pediatri, 10(6), 424-432.
Menteri Kesehatan Republik Indonesia. 2017. Peraturan Menteri Kesehatan Republik Indonesia Nomor 32 Tahun 2017 Tentang Standar Baku Mutu Kesehatan Lingkungan Dan Persyaratan Kesehatan Air Untuk Keperluan Higiene Sanitasi, Kolam Renang, Solus Per Aqua, Dan Pemandian Umum. Indonesia.
Ningsih N, Yasni S, Yuliani S. 2017. Sintesis nanopartikel ekstrak kulit manggis merah dan kajian sifat fungsional produk enkapsulasinya. Jurnal Teknologi dan Industri Pangan, 28(1), 27-35.
Parera, M. J., Supit, W., dan Rumampuk J. F. 2013. Analisis Perbedaan Pada Uji Kualitas Air Sumur Di Kelurahan Madidir Ure Kota Bitung Berdasarkan Parameter Fisika. Jurnal e-Biomedik. 1(1), 466-472.
Pertiwi RT, Djajadisastra J, Mutalib A, Pujiyanto A. 2019. Pembuatan, karakterisasi dan uji in vitro nanopartikel emas berbasis konjugat gom arab-vinkristin. Jurnal Ilmu Kefarmasian Indonesia, 16(01), 6-11.
Preet S, Sneha A. 2011. Biochemical evidence of efficacy of potash alum for the control of dengue vector Aedes aegypti (Linnaeus). Parasitology Research, 108(6), 1533–1539.
Rosidah I, Sumaryono W, Surini S. 2012. Preparasi mikrosfer fraksi etil asetat herba sambiloto (Andrographis paniculata Nees) dengan metode semprot kering. Jurnal Ilmu Kefarmasian Indonesia, 10(2), 132-137.
Salim AN, Sumardianto, Amalia U. 2018. Efektivitas serbuk simplisia biji pepaya sebagai antibakteri pada udang putih (Penaeus merguensis) selama penyimpanan dingin. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(2), 188-198.
Utami WW, Ahmad AR, Malik A. 2016. Uji aktivitas larvasida ekstrak daun jarak kepyar (Ricinus communis L.) terhadap larva nyamuk Aedes aegypti. Jurnal Fitofarmaka Indonesia, 3(1), 141–145.
Wahyuni D. 2016. Toksisitas Ekstrak Tanaman sebagai Dasar Biopeptisida Baru Pembasmi Larva Nyamuk Ades Aegepty (Ekstrak Dauan Sirih, Ekstrak Daun Biji Pepaya, dan Ekstrak Biji Srikaya) berdasar Hasil Penelitian. First Edition. Malang. Media Nusa Creative.
World Health Organization. 2005. Guidelines for Laboratory and field testing of mosquito larvacides. World Health Organization. https://apps.who.int/iris/handle/10665/69101
Yunilawati R, Yemirta, Arianita AC, Ardhanie SA, Hidayati N, Rahmi D. 2018. Optimasi proses spray drying pada enkapsulasi antosianin ubi ungu. Jurnal Kimia dan Kemasan, 40(1), 17-24.
Yuwono T, Binarjo A, Priyanti R. 2015. Pengembangan preparasi nanopartikel thymoquinone-kitosan dengan metode kosolven menggunakan isopropil alkohol. Pharmaciana, 5(2), 121–130.
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