Ekstraksi Kayu Artocarpus lakoocha: Pengaruh Metode Dan Rasio Bahan Tumbuhan-Penyari Terhadap Rendemen Ekstraksi
Abstract
Artocarpus lakoocha Roxb. (Ma-haad) is a medicinal plant commonly used in Thai Traditional Medicine (TTM) for a wide array of indications. In this study, we compared yield of extraction and the Thin Layer Chromatography (TLC) profile of the heartwood of A. lakoocha extracted with the different methods and different material-solvent ratios. Extractions were carried out by maceration and reflux extraction with ethanol as the solvent, in material-solvent ratio of 1:5 and 1:3. The TLC profile was obtained from the separation of the extracts with methylene chloride/methanol (85:15) on silica gel F254 plate. The result exhibited that reflux extraction produced the highest yield of extraction and the material-solvent ratio of 1:3 generated more yield than that of 1:5. Nevertheless, the profile of TLC chromatogram of those extracts were similar.
Keywords
References
Borah, H.J., Singhal, R., Hazarika, S. 2017. Artocarpus lakoocha Roxb.: an untapped bioresource of resveratrol from North East India, its extractive separation and antioxidant activity. Ind. Crop. Prod., 95:75–82.
Ćujić, N., Šavikin, K., Janković, T., Pljevljakušić, D., Zdunić, G., Ibrić, S. 2016. Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique. Food Chem., 194:135–142.
Jovanović, A.A., Đorđević, V.B., Zdunić, G.M., Pljevljakušić, D.S., Šavikin, K.P., Gođevac, D.M., Bugarski, B.M. 2017. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. Sep. Purif. Technol., 179:369–380.
Kumar, M.B.S., Kumar, M.C.R., Bharath, A.C., Kumar, H.R.V., Kekuda,T.R.P., Nandini, K.C., Rakshitha, M.N., Raghavendra, H.L. 2010. Screening of selected biological activities of Artocarpus lakoocha Roxb (Moraceae) fruit pericarp. J. Basic Clin. Pharm., 1:239–245.
Ledesma-Escobar, C.A., Priego-Capote, F., Luque de Castro, M.D. 2016. Comparative study of the effect of sample pretreatment and extraction on the determination of flavonoids from Lemon (Citrus limon). PLoS One, 11:e0148056.
Maneechai, S., De-eknamkul, W., Umehara, K., Noguchi, H. 2012. Flavonoid and stilbenoid production in callus cultures of Artocarpus lakoocha. Phytochemistry, 81:42–49.
Maneechai, S., Likhitwitayawuid, K., Sritularak, B., Palanuvej, C., Ruangrungsi, N., Sirisaard, P. 2009. Quantitative analysis of Oxyresveratrol content in Artocarpus lakoocha and “Puag-Haad.” Med. Princ. Pract., 18:223–227.
Mustafa, A., Turner, C. 2011. Pressurized liquid extraction as a green approach in food and herbal plants extraction: A review. Anal. Chim. Acta, 703:8–18.
Namdaung, U., Athipornchai, A., Khammee, T., Kuno, M. 2018. 2-Arylbenzofurans from Artocarpus lakoocha and methyl ether analogs with potent cholinesterase inhibitory activity. Eur. J. Med. Chem.,143:1301–1311.
Pandey, A., Bhatnagar, S.P. 2009. Preliminary phytochemical screening and antimicrobial studies on Artocarpus lakoocha Roxb. Anc. Sci. Life, 28:21–24.
Predescu, N.C., Papuc, C., Nicorescu, V., Gajaila, I., Petcu, G.V., Petcu, C.D., Stefan, G. 2016. The influence of solid-to-solvent ratio and extraction method on total phenolic content, flavonoid content and antioxidant properties of some ethanolic plant extracts. Rev. Chim. 67:1922–1927.
Preyavichyapugdee, N., Sangfuang, M., Chaiyapum, S., Sriburin, S., Pootaeng-on, Y., Chusongsang, P., Preyavichyapugdee, M., Sobhon, P. 2006. Schistosomicidal activity of the crude extract of Artocarpus lakoocha. Public Heal. Asian J. Trop. Med., 47:1–15.
Rezaei, S., Rezaei, K., Haghighi, M., Labbafi, M., 2013. Solvent and solvent to sample ratio as main parameters in the microwave-assisted extraction of polyphenolic compounds from apple pomace. Food Sci. Biotechnol., 22:1–6.
Sahne, F., Mohammadi, M., Najafpour, G.D., Moghadamnia, A.A. 2016. Extraction of bioactive compound curcumin from turmeric (Curcuma longa L.) via different routes: A comparative study. Pakistan J. Biotechnol. 13:173–180.
Saowakon, N., Tansatit, T., Wanichanon, C., Chanakul, W., Reutrakul, V. 2009. Fasciola gigantica: Anthelmintic effect of the aqueous extract of Artocarpus lakoocha. Exp. Parasitol., 122:289–298.
Sarker, S.D., Nahar, L. 2012. An Introduction to Natural Products Isolation, in: Sarker, S., Nahar, L. (Eds.), Natural Products Isolation: Methods in Molecular Biology (Methods and Protocols). New York: Humana Press, pp. 1–26.
Seidel, V., 2012. Initial and Bulk Extraction of Natural Products Isolation, in: Sarker, S., Nahar, L. (Eds.), Natural Products Isolation: Methods in Molecular Biology (Methods and Protocols). New York: Humana Press, pp. 27–41.
Shila, K., Pachauri, M., Kumar, A., Shukla, A., Patel, M., Jagannadham, M.V. 2015. Heme-peroxidase from medicinal plant Artocarpus lakoocha: Purification, characterization and wound healing studies. Biocatal. Agric. Biotechnol., 4:180–190.
Singh, J., 2008. Maceration, Percolation and Infusion Techniques for the Extraction of Medicinal and Aromatic Plants, in: Handa, S.S., Khanuja, S.P.S., Longo, G., Rakesh, D.D. (Eds.), Extraction Technologies for Medicinal and Aromatic Plants. Trieste: ICS-UNIDO, pp. 67–82.
Sritularak, B., Tantrakarnsakul, K.,Likhitwitayawuid, K., Lipipun, V. 2010. New 2-Arylbenzofurans from the root bark of Artocarpus lakoocha. Molecules, 15:6548–6558.
Wongkittipong, R., Prat, L., Damronglerd, S., Gourdon, C. 2004. Solid-liquid extraction of andrographolide from plants -experimental study, kinetic reaction and model. Sep. Purif.Technol., 40:147–154.
Wu, C., Wang, F., Liu, J., Zou, Y., Chen, X. 2015. A comparison of volatile fraction obtained from Lonicera macranthoides via different extraction processes: ultrasound, microwave, soxhlet extraction, hydrodistillation and cold-maceration. Integr. Med. Res., 4:171–177.
DOI: 10.30595/pharmacy.v15i1.2813
Copyright (c) 2018 Dwi Hartanti, Jirapat Theeravit
This work is licensed under a Creative Commons Attribution 4.0 International License.
ISSN: 2579-910X