Screening of Antibacterial Activity of Ethyl Acetate Fraction of Eleutherine palmifolia (L.) Merr Bulbs against Salmonella typhi
DOI:
https://doi.org/10.30595/pharmacy.v17i2.8999Keywords:
antibacterial, Eleutherine palmifolia (L.) Merr, ethyl acetate fraction, Salmonella thypiAbstract
Typhoid fever is an infection caused by the bacterium Salmonella typhi, spread through contaminated food or water. Typhoid fever can be treated with antibiotics, but at this time some pathogen microbes have been resistant to the available antibiotics. Antimicrobials derived from natural product might be the alternative to overcome antibiotic resistance. Eleutherine palmifolia (L.) Merr is empirically used to treat infectious diseases. E. palmifolia has chemical compounds of alkaloids, glycosides, flavonoids, phenolics, steroids, and tannins. This study aims to evaluate the antibacterial activity of ethyl acetate fraction against S. typhi. E. palmifolia bulbs were subsequently extracted using n-hexane solvents and followed by ethyl acetate solvents. The ethyl acetate fraction was tested for antibacterial activity using the disc diffusion method. The ethyl acetate fraction of E. palmifolia at the concentrations of 8, 6, and 8 mg/paper disc showed the diameters of the inhibition zone of 15.1±3.6, 15.3±3.3, and 16.9±1.9 mm, respectively. The conclusion of this study was the ethyl acetate fraction of E. palmifolia has a strong antibacterial activity against S. typhi.
References
Almeida, Y. (2014) Eleutherinone, a Novel Fungitoxic Naphthoquinone from Eleutherine bulbosa (Iridaceae), Memórias do Instituto Oswaldo Cruz, 95(5), pp. 709-712. doi: 10.1590/S0074-02762003000500021
Bajpai, V., Shukla, S. and Sharma, A. (2013) ‘Natural products: Phytochemistry, botany and metabolism of alkaloids, phenolics and terpenes’, in Ramawat, K. and Merillon, J. (eds) Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes. Springer-Verlag Berlin Heidelberg, pp. 3975–3988. doi: 10.1007/978-3-642-22144-6.
Cowan, M. M. (1999) Plant Products as Antimicrobial Agents, CLINICAL MICROBIOLOGY REVIEWS. Available at: http://cmr.asm.org/ (Accessed: 13 September 2020).
Fransira, I., Yanuhar, U. and Maftuch (2019) ‘Potential of Dayak Onion (Eleutherine palmifolia (L) Merr) Extract As Antibacterial Against Pseudomonas fluorescens’, The Journal of Experimental Life Science, 9(2), pp. 76–80. Available at: https://jels.ub.ac.id/index.php/jels/article/view/347 (Accessed: 13 September 2020).
Górniak, I., Bartoszewski, R. and Króliczewski, J. (2019) ‘Comprehensive review of antimicrobial activities of plant flavonoids’, Phytochemistry Reviews. Springer Netherlands, pp. 241–272. doi: 10.1007/s11101-018-9591-z.
Harborne, J., Padmawinata, K. and Soediro, I. (1987) Metode fitokimia: penuntun cara modern menganalisis tumbuhan (Phytochemical method: A modern guide to analyse plants). 2nd edn. Bandung: ITB Publisher.
Harlita, T., Oedjijono and Asnani, A. (2018) ‘The antibacterial activity of dayak onion (Eleutherine palmifolia (L.) merr) towards pathogenic bacteria’, Tropical Life Sciences Research, 29(2), pp. 39–52. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6072726/ (Accessed: 11 September 2020).
Jiang, H. et al. (2020) ‘The chemical constituents from the active fractions of Eleutherine bulbosa with their antimicrobial activity’, Natural Product Research. Taylor and Francis Ltd., 34(12), pp. 1743–1749. doi: 10.1080/14786419.2018.1530229.
Juwita, S., Hartoyo, E. and Budiarti, L. Y. (2013) Pola Sensitivitas In Vitro…, Berkala Kedokteran. Available at: https://ppjp.ulm.ac.id/journal/index.php/jbk/article/view/915 (Accessed: 12 September 2020).
Kelanit, R. S., Runtuboi, D. Y. P. and Gunaedi, D. T. (2016) ‘Uji Resistensi Antibiotik dan Deteksi Gen Plasmid IncHI1 Salmonella typhi Isolat Jayapura’, ejournal.uncen.ac.id, 8(1), pp. 48–56. Available at: http://ejournal.uncen.ac.id/index.php/JBP/article/view/47 (Accessed: 12 September 2020).
Li, L. et al. (2016) ‘The antibacterial activity and action mechanism of emodin from Polygonum cuspidatum against Haemophilus parasuis in vitro’, Microbiological research, pp. 139–145. Available at: https://www.sciencedirect.com/science/article/pii/S0944501316300842 (Accessed: 13 September 2020).
Limantara, S. et al. (2020) ‘Interaction Prediction of Compounds Contained in Eleutherine Palmifolia with Serotonin, Norepinephrine, and BDNF Receptors by Computation’, sysrevpharm.org, 11(3), pp. 765–772. Available at: http://www.sysrevpharm.org/?mno=117925 (Accessed: 12 September 2020).
Mahmudah, S., Muntaha, A. and Muhlisisn, A. (2019) ‘Effectiveness of Dayak (Eleutherine palmifollia (L) Merr) Extracts Against Escherichia coli In Vitro’, Tropical Health and Medical Research, 1(2), pp. 44–48. Available at: http://www.medlabtechnojournal.com/index.php/THMR/article/view/8 (Accessed: 13 September 2020).
Mutiah, R. et al. (2019) ‘Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS’, Traditional Medicine Journal, 24(3), pp. 139–159. doi: 10.22146/mot.44883.
Pervaiz, A. et al. (2016) ‘Alkaloids: an emerging antibacterial modality against methicillin resistant Staphylococcus aureus’, Current Pharmaceutical Design, 22(28), pp. 4420–4429. Available at: https://www.ingentaconnect.com/content/ben/cpd/2016/00000022/00000028/art00007 (Accessed: 13 September 2020).
WHO Situation Report (2018) ‘Typhoid’, 31 January. Available at: https://www.who.int/news-room/fact-sheets/detail/typhoid.
Wicaksono, I., Runadi, D. and Firmansyah, I. (2018) ‘Antibacterial activity test of dayak onions (Eleutherine palmifolia L. Merr.) ethanolic extract against Shigella dysenteriae ATCC 13313’, National Journal of Physiology Pharmacy and Pharmacology, 8(5), pp. 741–744. Available at: http://njppp.com/?mno=286328 (Accessed: 13 September 2020).
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