The Effect of Black Cumin Seed Oil on Alanine Aminotransferase Levels which are Influenced by Nutritional Status in Active Smokers
DOI:
https://doi.org/10.30595/pharmacy.v18i2.13256Keywords:
active smokers, ALT, BCSO, folic acid, randomized controlled trial, vitamin CAbstract
Black cumin seed oil (BCSO) has the main compound thymoquinone which is thought to have hepatoprotective activity through an antioxidant mechanism. Exposure to cigarette smoke can cause cellular oxidative stress which is the key to the inflammatory process of liver cells and an increase in the activity of the Alanine aminotransferase (ALT) enzyme. Vitamin C and folic acid are nutrients that affect ALT levels through antioxidant activity. The main objective of this study was to see the effect of BCSO administration on ALT levels which were influenced by the intake of vitamin C and folic acid in healthy respondents who were active smokers for 30 days. This study used a single-blind Randomized Controlled Trial (RCT) method, with a total of 39 test subjects divided into four groups. Group 1 received 3x1 placebo, group 2 received BCSO 3x1, group 3 received BCSO 3x2, group 4 received BCSO 3x3. Investigation based on the ANOVA test, it turned out that there was no significant difference in the mean ALT levels in both the placebo group and the treatment group with p value = 0.452. For the p value of the intake of vitamin C and folic acid were 0.359 and 0.669, respectively. Based on the results of the study showed that the administration of BCSO for 30 days had no significant effect on the average activity of ALT in healthy active smokers and there was no significant difference in the intake of vitamin C and folic acid.
References
ADA (American Diabetes Association). 2019. Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes. Diabetes Care, 42(1):13-28.
Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Anwar, F. 2013. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5):337–352.
Aji, A., Maulinda, L., Amin, S. 2015, Isolasi nikotin dari puntung rokok sebagai insektisida. Jurnal Teknologi Kimia Unimal, 4(1): 100 – 120.
Akrom. 2013. Mekanisme kemopreventif mbjh pada tikus Sprague Dawley (SD) yang diinduksi 7,12 dimethylbenza (A) antracene (DMBA). Disertasi. Yogyakarta: Pasca Sarjana, UGM.
Akrom, A., Darmawan, E., Yuhelvia, L. 2015. Black cumin seed oil as hepatoprotector in decreasing ALT and SGOT activity and increasing p53 gene expression in sprague dawley rats induced by alloxan. International Journal of Public Health Science (IJPHS), 4(3):159-163.
Alsaif, M.A. 2007. Effect of thymoquinone on ethanol-induced hepatotoxicity in wistar rats. Journal of Medical Sciences, 7: 1164-1170.
Baratawidjaja, K.G. 2014. Imunologi Dasar. Edisi Keempat. Jakarta: Balai Penerbit Fakultas kedokteran UI.
El-Dakhakhny, M., Madi, N., Lembert, N., Ammon, H. 2002. Nigella sativa oil, nigellone, and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorpho nuclear leukocytes from rats. Journal of ethnopharmacology, 81:161–164.
Fauzi, T.M. 2008. Pengaruh pemberian timbal asetat dan vitamin C terhadap kadar malondialdehyde dan kualitas spermatozoa di dalam sekresi epididimis mencit albino (Mus musculus L) strain BALB/C. Tesis. Medan: Universitas Sumatra Utara.
Gowing, L.R., Ali, R.L., Allsop, S., Marsden, J., Turf, E.E., West, R., Witton, J. 2015. Global statistics on addictive behaviours: 2014 status report. Addiction, 110(6):904–919.
Hariyatmi, 2004. Kemampuan vitamin E sebagai antioksidan terhadap radikal bebas pada usia lanjut. Jurnal MIPA, 14(1):54.
Juwita, R. 2011. Pengaruh pemberian minyak biji jinten hitam (Nigella sativa) terhadap aktivitas ALT dan AST pada tikus putih (Rattus novegicus) model hepatotoksik (etanol). Skripsi. Purwokerto: Universitas Jendral Soedirman.
Kresno, S.B. 2013. Imunologi: Diagnosis dan Prosedur Laboratorium. Edisi V. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia.
Loria, C.M., Ingram, D.D., Feldman, J.J., Wright, J.D., Madans, J.H. 2000. Serum folate and cardiovascular disease mortality among US men and women. Archives of Internal Medicine, 160:3258–3262.
Mahaboob, S.R., Jayarami R.U., John B.S. 2013. A study on serum enzyme levels in various liverhealth conditions. International Journal of Medical Research & Health Sciences, 2(3):395-398.
Oktavianti, N. 2015. Gambaran ekspresi gen interferon- γ (IFN- γ) dan aktivitas SGOT-ALT pada Relawan sehat yang diberi minyak biji jinten hitam (MBJH). Tesis. Yogyakarta: Program Studi Pascasarjana Farmasi, Universitas Ahmad Dahlan.
Restutiwati, E. Murbawani, Rahadiyanti, A. 2019. Kualitas diet, aktivitas fisik, dan status gizi pada perokok dewasa awal. Journal of Nutrition College, 8(3):156-163.
Sarna, L.K., Wu, N., Wang, P., Hwang, S. Y., Siow, Y.L., Karin, O. 2012. Folic acid supplementation attenuates high fat diet induced hepatic oxidative stress via regulation of NADPH oxidase. Canadian Journal of Physiology and Pharmacology, 90:155–165.
Setiawati, A., Suyatna, F.D., Gan, S. 2007. Pengantar Farmakologi. In: Gunawan, S.G., Setiabudy, R., Nafrialdi, Elysabeth. Farmakologi dan terapi. 5th ed. Jakarta: Departemen Farmakologi dan Teraupetik Fakultas Kedokteran Universitas Indonesia.
Sid, V., Wu, N., Sarna, L.K., Siow, Y.L., House, J.D. and Karmin, O. 2015. Folic acid supplementation during high-fat diet feeding restores AMPK activation via an AMP-LKB1-dependent mechanism. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 309(10):R1215–R1225.
Simanjuntak, L. 2010. Pengaruh Pemberian vitamin C terhadap gambaran histologis hati mencit (Mus musculus L) yang dipapari monosodium glutamate. Tesis. Medan: Fakultas Kedokteran, Universitas Sumatera Utara.
Sulistyowati, Y. 2006. Pengaruh pemberian likopen terhadap status antioksidan (vitamin C, vitamin E dan gluthation peroksidase) tikus (Rattus norvegicus galur sprague dawley) hiperkolesterolemik. Tesis. Semarang: Fakultas Kedokteran, Universitas Dipenogoro.
Syahrizal, D. 2008. Pengaruh proteksi vitamin C terhadap enzim transaminase dan gambaran histopatologis hati mencit yang dipapar plumbum. Tesis. Medan: Fakultas Kedokteran, Universitas Sumatera Utara.
Takami, T., Saito, Y. 2011. Effects of smoking cessation on central blood pressure and arterial stiffness. Vascular Health and Risk Management, 7:633–638.
Tanoeisan, A.P., Mewo Y.M., dan. Kaligis, S.H.M. 2016. Gambaran kadar serum glutamic pyruvic transaminase (ALT) pada perokok aktif usia > 40 Tahun. Skripsi. Manado: Universitas Sam Ratulangi Manado.
West, R. 2017. Tobacco smoking : health impact , prevalence, correlates and interventions interventions. Psychology & Health, 32(8):1018-1036.
Zhong, C.-Y., Zhou, Y.M., Pinkerton, K.E. 2008. NF-κB inhibition is involved in tobacco smoke-induced apoptosis in the lungs of rats. Toxicology and Applied Pharmacology, 230(2):150–158.Downloads
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